患者的ELISA一步法检测乙型肝炎抗—HBc,抗—HBe存在假阳性

Study of false positive reaction in determination of serum anti-HBc and anti-HBe by one-step ELISA

To certify the higher false positive reaction rate of serum anti-HBc and anti-HBe, 166 clinical specimensand 266 physical examing specimens were detected by one-step and two-step Enzyme Linked Immuno Sorbent Assay(ELISA) respectively with national reagents. Besides,the ABBOTT reagent was also used. Calculated by comparing thetwo reagents,the relative specificity of 166 clinical specimens and 266 physicaI examing specimens were 50K and 67. 7%for anti-HBc, 89. 3% and 88. 6% f0r anti-HBe. That determined by two-step ELISA were 98. 2% and 97. 5% for anti-HBc, 98. 8% and 99- 2% for anti-HBe respectively. The deference between national reagents and the ABBOTT reagentwere 30% in clinical specimens and 16% in physical examing specimens for anti-HBc, 12. 2% and 11. 9% for anti-HBe.The relative specificity of one-step is lower than that of two-step method,There is higher fales positive reaction in de-tecting anti-HBc and anti-HBe by one-step ELISA.