阿托伐他汀对脂多糖诱导的人肺上皮细胞分泌PGE2和IL-6的影响

目的：探讨阿托伐他汀（atorvastatin）对经脂多糖（LPS）诱导人肺上皮细胞(A549)的炎症细胞因子PGE2和IL-6分泌的影响。方法：对LPS诱导的人肺上皮细胞给予不同浓度的阿托伐他汀干预，同时设置对照组，分别收集其培养上清液，采用ELISA方法，比较PGE2和IL-6浓度。结果：①在不同浓度的LPS诱导下，肺泡上皮细胞不同时间分泌PGE2和IL-6浓度明显高于未经LPS诱导的对照组（P<0.05）。②阿托伐他汀不同浓度的干预，肺上皮细胞PGE2和IL-6的分泌浓度较无阿托伐他汀干预组均有不同程度的降低（P<0.05）。结论：阿托伐他汀能降低脂多糖诱导的人肺上皮细胞炎症因子PGE2和IL-6的分泌, 提示他汀类药物对肺上皮细胞有直接抗炎作用。

Effects of atorvastatin on LPS-induced PGE2 and IL-6 secretionsin human pulmonary epithelial cells

Objective To investigate the effects of atorvastatin on LPS induced PGE 2 and IL 6 secretions in human pulmonary epithelial cells (A549). Methods The supernates of LPS induced cultured human pulmonary epithelial cells from different intervention groups were collected to measure the concentrations of PGE 2 and IL 6 by enzyme linked immunosorbent assay.Results The concentrations of PGE 2 and IL 6 in supernates of LPS induced human pulmonary epithelial cells were significantly higher than those in the control group ( P <0.05) at different time points.The concentrations of PGE 2 and IL 6 in supernates of atorvastatin intervention groups at different levels were significantly lower than those in non intervention group ( P <0.05).Conclusion Atorvastatin decreased LPS induced PGE 2 and IL 6 secretions in human pulmonary epithelial cells (A549),which suggests that statins have anti inflammatory properties on human pulmonary epithelial cells.