HPLC法同时测定射干提取物多组分含量

目的：建立同时测定射干提取物中鸢尾苷、野鸢尾苷、鸢尾黄素、野鸢尾黄素、次野鸢尾黄素及白射干素6种有效成分含量的HPLC法。方法：采用KromasilC18色谱柱（5μm,4.6mm×200mm）,流动相为乙腈和0.5%磷酸水溶液,梯度洗脱,流速1.0mL/min;检测波265nm。结果：鸢尾苷、野鸢尾苷、鸢尾黄素、野鸢尾黄素、次野鸢尾黄素及白射干素线性范围分别是：0.3188~1.9129μg（r=0.9910）,0.1156~0.5782μg（r=0.9998）,0.0633~0.3164μg（r=0.9990）,0.4146~2.0730μg（r=0.9991）,0.0818~0.4091μg（r=0.991）,0.0458~0.2291μg（r=0.9990）,对照品线性关系良好,各对照品的平均回收率分别为：97.44%、98.29%、97.41%、98.01%、98.34%、96.73%。结论：该方法准确、可靠、重现性好可用于射干提取物的质量控制。

Determination of Multiple Constituents in Ethanol Extract of Bdamcanda Chinensis by HPLC

Objective： The research aimed to establish a method for the content determination of six kinds of active components such as tectoridin, iridin, teetorigenin, irigenin irisflorentin and dichotomitin. The contents of active compontents were determined by HPLC. Method ： HPLC method was established with Kromasil Cls column （ 5 μ m ,4.6 mm x 200 mm ） and a mobile phase of acetonitrile- 0.5% phosphoric acid. The flow rate was 1.0 mL/min, the detection wavelength was 265 nm and the column temperature was 25 ℃ . Result ： The linearity ranges of tectoridin, iridin, tectorigenin, irigenin, irisflorentin and dichotomitin were 0.3188btgto 1.91291＊g （r=0.9910）,0.1156 μgto 0.5782 μg （r=0.9998）,0.0633 μgto 0.3164 μg （r=0.9990）,0.4146 μg to 2.0730 μg （r=0.9991）,0.0818 μg to 0.4091 μg （r=0.991）and 0.0458 μg to 0.2291 μ g （ r=0.9990 ）, respectively.The average recoveries （ n=5 ）were 97.44%, 98.29%, 97.41%, 98.01%, 98.34% and 96.73%. Conclusion ： This method is accurate, reliable and with good repro-dueibility. It can be used for the quality control of the ethanol extract of Belameanda chinensis.