Antigen-coupled beads adherent to slides: a simplified method for immunological studies. |
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Authors: | J G Streefkerk A M Deelder N Kors D Kornelis |
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Affiliation: | 1. Department of Histology, Free University, van der Boechorststraat 7, Amsterdam, The Netherlands;7. Laboratory of Parasitology, University of Leiden, Rapenburg 33, Leiden, The Netherlands |
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Abstract: | Small amounts of antigen-coupled beads adherent to object slides provide a simple, quick, economical and sensitive immunohistochemical means of detecting antibodies in serum by both immunofluorescence and immunohistoperoxidase procedures. Sensitivity increases with decreasing quantities of antigen-coupled beads, as was demonstrated in the fluorescence procedure. |
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