| 肥胖对小鼠十二指肠DMT1和FPN1表达的影响 |
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| 引用本文： | 高倩,李蔓,张万山,魏守刚.肥胖对小鼠十二指肠DMT1和FPN1表达的影响[J].中国比较医学杂志,2014,24(9):18-22. |
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| 作者姓名： | 高倩 李蔓 张万山 魏守刚 |
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| 作者单位： | 首都医科大学公共卫生学院儿少卫生与妇幼保健学系，北京 100069; 首都医科大学，环境毒理学北京市重点实验室，北京 100069 |
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| 基金项目： | 国家自然科学基金项目（81373020）；北京自然科学基金项目（7112014）。 |
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| 摘 要： | 目的观察肥胖对小鼠十二指肠二价金属离子转运体(divalent metal transporter 1,DMT1)mRNA、膜铁转运蛋白(ferroportin1,FPN1)mRNA及蛋白表达的变化,探讨肥胖影响铁吸收的机制。方法 C57BL/6J小鼠随机分为正常对照组和肥胖模型组,每组6只,通过喂养高脂饲料喂养建立肥胖模型,对照组采用普通饲料饲养,实验干预期14周。建模完成后,采用实时荧光定量PCR方法检测小鼠十二指肠DMT1、FPN1 mRNA的表达,用Western blot检测小鼠十二指肠FPN1蛋白表达。结果与对照组小鼠相比,肥胖模型组小鼠十二指肠DMT1、FPN1mRNA表达以及FPN1蛋白表达水平降低,差异具有统计学意义(P0.05)。结论肥胖会下调机体十二指肠DMT1、FPN1的表达,导致铁吸收不良,为进一步研究肥胖引起铁缺乏机制提供理论和实验依据。
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| 关 键 词： | 肥胖 铁代谢 二价金属离子转运体 膜铁转运蛋白 |
| 收稿时间： | 2014/6/18 0:00:00 |
| 修稿时间： | 2014/8/25 0:00:00 |
Effect of obesity on the expression of DMT1 and FPN1 in the duodenal in mice |
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| gao qian,li man,zhang wan shan and wei shou gang.Effect of obesity on the expression of DMT1 and FPN1 in the duodenal in mice[J].Chinese Journal of Comparative Medicine,2014,24(9):18-22. |
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| Authors: | gao qian li man zhang wan shan and wei shou gang |
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| Institution: | Department of child and adolescent health and maternal care, School of Public Health, Capital Medical University, Beijing 100069, China;Beijing Key Laboratory of Environmental Toxicology, Capital Medical University, Beijing 100069, China;Department of child and adolescent health and maternal care, School of Public Health, Capital Medical University, Beijing 100069, China;Beijing Key Laboratory of Environmental Toxicology, Capital Medical University, Beijing 100069, China;Department of child and adolescent health and maternal care, School of Public Health, Capital Medical University, Beijing 100069, China;Beijing Key Laboratory of Environmental Toxicology, Capital Medical University, Beijing 100069, China;Department of child and adolescent health and maternal care, School of Public Health, Capital Medical University, Beijing 100069, China;Beijing Key Laboratory of Environmental Toxicology, Capital Medical University, Beijing 100069, China |
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| Abstract: | |
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| Keywords: | Obesity Iron metabolism Divalent metal transporter 1 Ferroportin 1 |
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