WIN 55,22-2对大鼠局灶性脑缺血再灌注损伤的保护作用

目的:探讨大麻素(CB)受体激动剂WIN55,212-2对大鼠局灶性脑缺血的保护作用。方法:采用大鼠大脑中动脉栓塞(MCAO)致局灶性脑缺血模型。将50只雄性SD大鼠随机分为5组:对照组(Con组)于MCAO前30 min腹腔注射生理盐水0.3 ml;WIN55,212-2组(WIN1~3组)于MCAO前30 min腹腔注射WIN55,212-2 0.3,1和3 mg/kg;DMSO组于MCAO前30 min腹腔注射二甲基亚砜(DMSO)0.3 ml。观察MCAO120 min再灌注24 h后神经功能评分(NFS)和脑梗死容积百分比。结果:与DMSO组和Con组比较,WIN55,212-2组大鼠NFS明显升高(P<0.05),脑梗死容积百分比明显减小(P<0.05)。结论:CB受体激动剂WIN55,212-2对大鼠局灶性脑缺血再灌注损伤具有保护作用,并具有一定的剂量相关性。

Neuroprotective effect of cannabinoid receptor agonist WIN 55,212-2 on focal cerebral ischemia in rats

Objective:To investigate the neuroprotective effect of cannabinoid receptor agonist WIN 55,212-2 on focal cerebral ischemia in rats.Methods:Focal cerebral ischemia was induced by the middle cerebral artery occlusion(MCAO) for 120 min.Fifty male SD rats were randomly assigned to five groups(n=10 each) as follows: The control group(Con) were intraperitoneally injected normal sodium 0.3 ml 30 min before MCAO;DMSO group was intraperitoneally injected dimethyl sulfoxide(DMSO) 0.3 ml 30 min before MCAO;WIN 55,212-2 groups in which WIN 55,212-2 was intraperitoneally injected at the doses of 0.3,1 or 3 mg/kg respectively 30 min before MCAO.The neurological function scores(NFS) and brain infarct volumes were evaluated at 24 h after reperfusion.Results: Compared with the control group and DMSO group,the NFS in WIN 55,212-2 groups was significantly increased(P<0.05),while the infarct volume in WIN 55,212-2 groups was significantly decreased(P<0.05) at 24 h after reperfusion.Conclusion: WIN 55,212-2 has neuroprotective effect on focal cerebral ischemia with a dose-dependent manner in rats.