基于大鼠体内序贯代谢的白芍质控成分遴选研究

目的 基于序贯代谢的研究思路，研究白芍Paeoniae Radix Alba多种成分在体内的代谢情况，遴选白芍潜在质控成分。方法 制备白芍水提物，通过在体动物实验的方法，收集白芍的肠代谢、肝代谢以及综合代谢样品，结合超高效液相色谱-四级杆/静电场高分辨质谱联用技术（UPLC-Q-Exacitve MS），对各个样品所含成分进行分析与鉴别。结果 白芍水提物中共鉴定出36个化学成分。序贯代谢结果显示，共有17个原型入血成分，其中有14个成分在经肠代谢样品中检测到，有8个成分先经肠代谢、再经肝代谢后可检测到，有11个成分在经ig给药的综合代谢样品中检测到；羟基芍药苷、芍药苷、芍药内酯苷、环五（异）亮氨酸、环六（异）亮氨酸、苯甲酸、对羟基苯甲酸这7个成分在3种代谢部位均可以检测到。结论 共遴选出没食子酸、芍药内酯苷等17个经代谢后仍原型入血的成分，可作为日后白芍质控成分。

Selection of quality control components from Paeoniae Radix Alba based on sequential metabolism in rats

Objective To investigate the metabolism of Radix Paeoniae Alba and to select potential quality control components based on the research through of sequential metabolism. Methods After preparing the water extract of Radix Paeoniae Alba, in vivo animal experiments were applied to collect its intestinal metabolism, liver metabolism, as well as comprehensive metabolism samples, combined with ultra-high performance liquid chromatography-quadrupole/electrostatic field high-resolution mass spectrometry(UPLC-Q-Exactive MS), to analyze and identify the components of samples. Results A total of 36 chemical components were identified from the water extract of Radix Paeoniae Alba. Sequential metabolic studies shown that there were 17 protoplasmic components, of which 14 components could be detected after intestinal metabolism, 8 components could be detected after intestinal metabolism followed by liver metabolism, and 11 components could be detected in comprehensive metabolic samples after intragastric administration. Hydroxypaeoniflorin, paeoniflorin, paeoniflorin, cyclopenta (isoleucine), cyclohexa (isoleucine), benzoic acid and p-hydroxybenzoic acid were detected in the three metabolic sites. Conclusion Through analyzing the results of different samples, 17 components that still can enter the blood circulation in the form of a prototype after metabolism, such as gallic acid, albiflorin and so on, were selected as potential quality control components of Radix Paeoniae Alba in the future.