粗茎秦艽多糖GCP-1的分离纯化、结构表征及抗炎活性评价

目的 从粗茎秦艽Gentiana crassicaulis中分离纯化多糖,初步分析其结构特征并研究其体外抗炎活性.方法 采用热水浸提粗茎秦艽粗多糖(Gentiana crassicaulis polysaccharide,GCP),再经Cellulose DE-52纤维素离子交换柱、SephadexG-100柱进行分离...

Isolation, purification, structure characterization, and anti-inflammatory activity of Gentiana crassicaulis polysaccharides

Objective Gentiana crassicaulis polysaccharides(GCP-1)was extracted and purified to study the structural features and anti-inflammatory activity in vitro.Methods The crude polysaccharides of Gentiana crassicaulis(GCP)was extracted by hot water.The Cellulose DE-52 and Sephadex G-100 columns were used to separate and purify homogeneous polysaccharides.The relative molecular mass was analyzed by high-performance gel permeation chromatography,and the monosaccharide composition and structure were preliminarily identified by HPLC,IR,GC-MS,and NMR.The effects on proliferation function of RAW 264.7 cells were determined by CCK-8,and the release capacity of IL-1β,NO,and TNF-αwere determined by the ELISA kit.Results The molecular weights of the GCP-1 polysaccharides extracted and purified from G.crassicaulis was 6.87×10⁴.GCP-1 was consisted of mannose,rhamnose,glucuronic acid,galacturonic acid,glucose,galactose and arabinose.The molar ratios of these monosaccharide were 0.07∶3.07∶0.42∶1.98∶1.33∶5.22∶6.76.The structure of GCP-1 was characterized by IR,GC-MS and NMR,and GCP-1 was a typical pectin polysaccharide,with HG region,RG-I region and AG-I/AG-II side chains.When the concentration was 10-200μg/m L,GCP-1 significantly promoted the proliferation of RAW 264.7 cells(P<0.05)and inhibited the release of IL-1β,NO,and TNF-α.Conclusion GCP-1 is a kind of natural homogeneous polysaccharides.Structural characterization and anti-inflammatory activity of GCP-1 provide the significant reference for the further development and elucidation of G.crassicaulis polysaccharides.