基于UPLC-Q-TOF-MS技术的芪风固表颗粒血清药物化学研究

目的运用中药血清药物化学方法初步分析大鼠口服给药芪风固表颗粒后的入血成分。方法采用UPLC-Q-TOF-MS技术进行分析,结合Peakview和Metabolitepilot软件,根据所提供的保留时间、精确相对分子质量及二级质谱裂解碎片,鉴定和表征其血清中化学成分。结果在给药后的大鼠血清中分析表征了37个化学成分,其中17个原型成分、20个代谢产物。结论借助UPLC-Q-TOF-MS方法鉴定了大鼠口服芪风固表颗粒后血清中的化学成分,初步分析了芪风固表颗粒的入血成分,为阐明该方的药效物质基础提供依据。

Study on serum pharmacochemistry of Qifeng Gubiao Granules based on UPLC-Q-TOF-MS

Objective To preliminarily analyze the components in rat serum after oral administration of Qifeng Gubiao Granules(芪风固表颗粒)by serum pharmacochemical methods.Methods The rat serum were collected and analyzed by UPLC-Q-TOF-MS after intragastric administration of Qifeng Gubiao Granules.The chemical components in control and dose groups were identified and speculated by Peakview and Metabolite Pilot data processing software using retention time,exact relative molecular mass,and cleavage fragments of MS/MS as indexes.Results A total of 37 chemical components,including 17 prototype components and 20 metabolites,were analyzed and characterized in rat serum after administration.Conclusion The chemical constituents in the rat serum were identified after oral administration of Qifeng Gubiao Granules based on UPLC-Q-TOF-MS,and the components in serum of Qifeng Gubiao Granules were preliminarily analyzed,which could clarify its pharmacodynamic material basis and provide evidence for clarify its pharmacodynamic material basis.