氨基酸转化三七花中稀有人参皂苷及提取液对脂多糖诱导的RAW264.7细胞活性的影响

目的通过添加食品级氨基酸将三七Panaxnotoginseng花中普通人参皂苷转化为稀有人参皂苷,并对转化前后提取物的抗炎活性进行比较。方法应用单因素和正交试验考察了不同氨基酸种类、氨基酸浓度、反应温度、反应时间和液固比对稀有人参皂苷转化的影响,探讨并分析了转化前后三七花提取物对脂多糖(LPS)诱导RAW264.7细胞增殖和抗炎活性的影响。结果天冬氨酸是最佳催化剂,在温度为120℃、天冬氨酸为5%、液固比为30m L/g和反应时间为2h时,4种稀有人参皂苷总转化含量为(47.12±0.52)mg/g。转化前后三七花提取物在1.56~200μg/m L,显著抑制了LPS诱导的RAW264.7细胞的活性(P<0.05),显著抑制NO和白细胞介素-6(IL-6)的释放(P<0.05)。在质量浓度≥6.250μg/m L时,转化后三七花提取物显著对LPS诱导RAW264.7细胞炎症有更好的抑制作用(P<0.05),NO和IL-6促炎因子的分泌也显著减少(P<0.05)。结论对稀有人参皂苷的转化提供了绿色、安全和高效的转化方法。三七花在转化后对LPS诱导的炎症反应有明显的抑制作用,为三七花的开发利用提供了新思路。

Amino acid conversion of rare ginsenosides in Panax notoginseng flower and its effect on RAW264.7 cell viability induced by lipopolysaccharide

Objective In this study,the common ginsenosides in Panax notoginseng flower(PNF)were converted into rare ginsenosides by adding food-grade amino acids,and the anti-inflammatory activities of the extracts before and after the transformation were compared.Methods Single factor and orthogonal experiments were used to investigate the effects of different amino acid types,amino acid concentration,reaction temperature,reaction time and liquid-solid ratio on the conversion of rare ginsenosides.The effects of PNF extracts before and after research transformation on the proliferation and anti-inflammatory activity of RAW264.7 cells induced by lipopolysaccharide(LPS)were analyzed.Results Aspartic acid was the best catalyst.When the temperature was 120℃,aspartic acid was 5%,the liquid-solid ratio was 30 m L/g,and the reaction time was 2 h,the total conversion of four rare ginsenosides was(47.12±0.52)mg/g.The PNF extract before and after transformation significantly inhibited the activity of RAW264.7 cells induced by LPS in the range of 1.56—200μg/m L(P<0.05),and significantly inhibited the release of NO and IL-6(P<0.05).When the concentration was greater than 6.250μg/m L,PNF extract after transformation significantly inhibited LPS-induced inflammation of RAW264.7 cells(P<0.05),and the secretion of NO and IL-6 pro-inflammatory factors was also significantly reduced(P<0.05).Conclusion This article provides a green,safe and efficient transformation method for the transformation of rare ginsenosides PNF has a significant inhibitory effect on the inflammatory response induced by LPS after transformation,which provides a new idea for the development and utilization of PNF.