基于网络药理学研究小儿升麻方对流感病毒性肺炎小鼠的治疗作用机制

目的 研究小儿升麻方对流感病毒性肺炎小鼠的治疗作用,并通过网络药理学方法探究其抗流感病毒性肺炎的作用机制.方法 小鼠随机分为对照组,模型组,小儿升麻方低、中、高剂量(1.75、3.50、7.00 g/kg)组和生理盐水组,给药第2天,经鼻滴入0.03mL FM1流感病毒尿囊液建立流感病毒性肺炎模型,第7天测定小鼠肺指数...

Mechanism of Xiaoer Shengma Formula on influenza virus pneumonia mice based on network pharmacology

Objective To study the effect of Xiaoer Shengma Formula(小儿升麻方)on influenza virus pneumonia mice and explore its anti-influenza virus pneumonia mechanism through network pharmacology.Methods Mice were randomly divided into control group,model group,low-,medium-,high-dose Xiaoer Shengma Formula(1.75,3.50,7.00 g/kg)groups and normal saline group.On day 2 after administration,mice were intranasally injected with 0.03 m L FM1 influenza virus allantoic fluid to establish influenza virus pneumonia model.Lung index of mice were measured on day 7.Chemical composition and target information of Xiaoer Shengma Formula were searched from TCMIP database by using network pharmacology method,influenza virus pneumonia-related targets were searched from Genecards database,common targets between Xiaoer Shengma Formula and influenza virus pneumonia were screened through Venn diagram.Gene ontology(GO)functional enrichment analysis and Kyoto encyclopedia of genes and genomes(KEGG)signaling pathway analysis on common targets were performed by Omicsbean analysis system and STRING database.Core targets were visualized by combining STRING database and Cytoscape 3.7.0 software,andmedicinal materials-ingredients-targets-pathwaysnetwork was established.Results High-dose Xiaoer Shengma Formula group significantly reduced the lung index of mice with influenza virus pneumonia(P<0.05).There were 69 common targets between Xiaoer Shengma Formula and influenza virus,and 24 core targets were screened through protein-protein interaction(PPI)network,corresponding to 29 main chemical components and 86 main pathways.Conclusion Xiaoer Shengma Formula has a potential therapeutic effect on mice with influenza virus pneumonia,which could play an anti-influenza virus pneumonia effect through multiple components,multiple targets,and multiple pathways.