牛奶蛋白过敏婴儿血清IL-10及TGF-β水平的表达研究

目的 比较牛奶蛋白过敏婴儿血清IL-10及TGF-β水平,分析其在婴儿牛奶蛋白过敏中的作用。 方法 选取2018年1-6月在深圳市妇幼保健院住院经回避激发试验确诊为牛奶蛋白过敏患儿20例为牛奶蛋白过敏组,选取同期健康婴儿15例作为对照组,采用ELISA检测两组婴儿血清IL-10、TGF-β及IgG4水平,同时检测食物过敏原总IgE以及牛奶特异性IgE。 结果 牛奶蛋白过敏组总IgE＞200者5例,总IgE 100～200者2例,总IgE＜100者13例。血清IL-10、TGF-β及IgG4水平均较对照组降低(t=-3.130、-2.811、-3.463,P＜0.05)。在牛奶蛋白过敏组中,IgE阳性亚组与IgE阴性亚组IgG4水平比较,差异无统计学意义(t=-0.781,P＞0.05)。 结论 IL-10及TGF-β在婴儿牛奶蛋白过敏的发病中起重要作用,IgG4与IgE可能无关。动态观察IL-10及TGF-β水平变化,对尽早了解牛奶蛋白过敏婴儿疾病的转归或耐受有积极作用。

Expression of serum levels of interlukin-10 and transforming and growth factor-β in infants with cow′s milk protein allergy

Objective To study the roles of interlukin-10(IL-10) and transforming and growth factor-β(TGF-β) in pathogenesis of cow′s milk protein allergy(CMPA) in infants,in order to analyze the role of IL-10 and TGF-β in the process of cow′s milk protein allergy.Methods A total of 20 infants diagnosed with CMPA by oral food challenge(OFC) test in Shenzhen Maternity and Child Healthcare Hospital from January to June in 2018 were enrolled in CMPA group,meanwhile 15 healthy infants were selected as control group. Serum levels of IL-10,TGF-β and IgG4 were tested by enzyme-linked immunosobent assay,and total IgE and milk specific IgE were assayed as well. Results There were 5 cases with total IgE＞200,2 cases with total IgE between 100 and 200,13 cases with total IgE＜100 in CMPA group. Serum levels of IL-10,TGF-β and IgG4 in CMPA group were significantly lower than those of control group(t=-3.130,-2.811,-3.463,P＜0.05). CMPA group was divided into IgE positive sub-group and IgE negative sub-group,and there was no significant difference on IgG4 level between the two groups(t=-0.781,P＞0.05). Conclusions IL-10 and TGF-β play important roles in pathogenesis of CMPA in infants. On the contrary,there may be no significant correlation between IgG4 and IgE. It is necessary to know the prognosis and immunotolerance of infants with CMPA by dynamic observation of IL-10 and TGF-β.