人白细胞介素-2与乙型肝炎病毒前S抗原融合蛋白基因疫苗诱导抗前S抗体产生

为了构建抗乙型肝炎病毒（HBV）持续性感染的特异性免疫治疗剂，我们曾构建了人白细胞介素-2（IL－2）与HBV前S（preS）抗原的融合蛋白（IL－2preS）的真核表达克隆，并证明IL－2preS融合蛋白能在哺乳动物细胞中分泌表达。本文以该克隆为基因疫苗，经正常肌肉组织内接种和再生性肌肉组织内接种进行基因免疫，实验结果证明接种IL－2preS基因疫苗能在动物体内诱导抗preS抗体产生，免疫后15天可检出抗体，45天时达高峰值，60天不见下降。再生性肌肉内的免疫效果比正常肌肉的好，所产生的抗体水平前者显著高于后者。结果表明IL－2pres融合蛋白能在机体内分泌表达。提示IL－2preS基因疫苗在人体内可能有多重生物功能，如诱导体液免疫与细胞免疫应答，双重导向作用和细胞膜受体交联效应等。

Production of Antibodies against preS Antigen of Hepatitis B Virus Induced by Gene Vaccine Encoding the Fusion Protein Composed of Human Interleukin-2 and preS Antigen

In order to search for specific therapeutic drugs against persistant infection of hepatitis B virus (HBV),a eukaryotic expression clone for the fusion protein(IL-2 preS) consisting in human interleukin-2(IL-2) and preS antigen(preS) was previously constructed and it was confirmed that IL-2 preS could be secreted from mammalian cells. In this study, the expression clone was used as gene vaccine and was innoculated Balb/c mice through regenerating muscles and normal muscles. The experimental results showed that inoculation of IL-2 preS gene vaccine could induce the mice to produce antibodies against preS antigen. The antibody level could be detected 15 days post immunization,reached the highest level 45 days and didn't decrease 60 days. The regenerating muscular inoculation was more effective than the normal muscular inoculation and the antibody titerof the former was significantly higher than that of the latter. It indicated that IL-2 preS fusionprotein could be secreted in bodies,and suggested that IL-2 preS gene vaccine might be of multiple function,including induction of humoral and cellular immune responses,and double receptorlinking effects,in human bodies.