慢性吸烟大鼠肺组织FIZZ1/RELMα表达的变化

目的：研究吸烟大鼠肺组织中FIZZ1/RELMα表达的变化及其与气道炎症和气道高反应性的关系。 方法： 复制大鼠的慢性吸烟模型，采用气道反应性测定、HE染色、免疫组织化学染色、原位杂交等方法，研究慢性吸烟大鼠肺组织中FIZZ1/RELMα表达的变化。 结果： 在正常对照组，RELMα蛋白呈散在分布，吸烟组支气管平滑肌、肺血管壁、肺泡上皮和肺间质的表达明显增强，吸烟组RELMα mRNA的表达在各种细胞均明显增强，其中以肺泡上皮、肺动脉内皮和支气管平滑肌的表达增强最为明显。 结论： FIZZ1/RELMα作为肺部特异性表达的一种因子，吸烟使其表达增强，与吸烟引起的气道高反应性、肺血管反应性增强密切相关。

Chronic cigarette smoking induces alteration of FIZZ1/RELMα expression in rat lung

AIM: To investigate the expression of FIZZ1/RELMα in lung tissue of chronic cigarette smoking rat, and to determine the relationship between airway inflammation and airway hyperresponsiveness. METHODS: Made rat model of chronic cigarette smoking was used. The expression of FIZZ1/RELMα in lung tissue was determined by immuno-histochemistry and in situ hybridization. RESULTS: In control rats, FIZZ1/RELMα protein and mRNA expressions were observed at low levels. In cigarette smoking rats, FIZZ1/RELMα expression increased in all the cells especially in bronchial smooth muscle cells, vascular wall cells and alveolar epithelial cells. CONCLUSION: FIZZ1/RELMα is a secreted peptide specifically expressed in lung. Cigarette smoking induces its upregulation, which possibly contributes to cigarette smoking-induced airway hyperresponsiveness.