| 基因芯片技术检测子宫内膜异位症相关细胞因子及其受体基因的表达谱 |
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| 引用本文： | 樊杨,陈必良,马向东,苏明权. 基因芯片技术检测子宫内膜异位症相关细胞因子及其受体基因的表达谱[J]. 细胞与分子免疫学杂志, 2005, 21(4): 489-492 |
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| 作者姓名： | 樊杨 陈必良 马向东 苏明权 |
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| 作者单位： | 第四军医大学西京医院妇产科,陕西,西安,710032 |
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| 摘 要： | 目的:研究与子宫内膜异位症(EM)发生和发展相关的细胞因子(CK)基因的表达,进一步阐明子宫内膜异位症的发病机制。方法:应用含有1200条基因的基因芯片,用同位素探针标记,探讨EM组织与正常子宫组织相关CK基因的表达谱。结果:在3例EM组织与3例正常的子宫内膜组织中,共筛选出差异表达基因119条,其中CK及CKR基因表达上调15条,包括IL1、IL2、IL6、IL8、VEGFR、TGF、EGF、FGF和EPOR等。结论:表达差异基因有助于揭示EM发生发展的分子机制,基因表达谱芯片能有效地筛选EM相关的CK及CKR基因,为了解疾病发生机制提供了高效、准确的研究工具。
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| 关 键 词： | 子宫内膜异位症 细胞因子 基因芯片 基因表达 |
| 文章编号： | 1007-8738(2005)03-0489-04 |
| 修稿时间： | 2004-06-13 |
Detection of expression of endometriosis-related cytokine and their receptor genes by cDNA microarray technique |
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| FAN Yang,CHEN Bi-liang,MA Xiang-dong,SU Ming-Quan. Detection of expression of endometriosis-related cytokine and their receptor genes by cDNA microarray technique[J]. Chinese journal of cellular and molecular immunology, 2005, 21(4): 489-492 |
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| Authors: | FAN Yang CHEN Bi-liang MA Xiang-dong SU Ming-Quan |
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| Affiliation: | Department of Obstetrics and Gynecology, Xijing Hospital, Fourth Military Medical University, Xi'an 710032, China. |
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| Abstract: | AIM: To study the pathogenesis of endometriosis(EM) by investigating cytokine(CK) and CKR genes expression involved in the development of EM. METHODS: The CK and CKR gene expression pattern in samples of EM and normal endometrial tissues were analyzed by using cDNA microarrays. RESULTS: 119 genes were expressed differently between 3 cases EM tissues and 3 cases normal endometria tissues. Among them, 15 were CK and CKR genes, including IL-1, IL-2, IL-6, IL-8, VEGFR, TGF, EGF, FGF, and EPOR etc. CONCLUSION: These 15 differently expressed CK and CKR genes may be correlated with the development of EM. |
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| Keywords: | endometriosis cytokine cDNA microarray gene expression |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
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