| STUDY ON THE EXPRESSION OF CYCLOOXYGENASE-2 IN HEPATOCELLULAR CARCINOMA CELL LINES AND ON THE GROWTH INHIBITION EFFECT OF NS-398 |
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| 引用本文： | 王崑,邢宝才,张青云,徐光炜. STUDY ON THE EXPRESSION OF CYCLOOXYGENASE-2 IN HEPATOCELLULAR CARCINOMA CELL LINES AND ON THE GROWTH INHIBITION EFFECT OF NS-398[J]. 中国癌症研究, 2006, 18(1): 32-37. DOI: 10.1007/s11670-006-0032-y |
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| 作者姓名： | 王崑 邢宝才 张青云 徐光炜 |
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| 作者单位： | Department of Surgery, Peking University School of Oncology, Beijing Cancer Hospital,Beijing Institute for Cancer Research, Beijing 100036 |
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| 摘 要： | Objective： To investigate the expression of cyclooxygenase -2 （COX-2） in hepatocellular carcinoma cell lines and to explore the effect of NS-398, a selective inhibitor for COX-2, on HepG-2 cell line. Methods： lmmunohistochemistry and RT-PCR were used to investigate COX-2 expression in 6 HCC cell lines. MTT and Flowcytometry were used to evaluate the effect of the selective inhibitor of COX-2, NS-398, on HepG-2 cell lines. Results： All six HCC cell lines showed COX-2 expression at protein level. Five out of 6 cell lines showed COX-2 expression at mRNA level. NS-398 could suppress the growth of HepG-2 cell line, in a time and dose dependant manner. Conclusion： NS-398, a selective inhibitor of COX-2, showed inhibition effect on HepG-2 HCC cell line. The efficacy of inhibition was time and dose dependent, providing a new evidence for chemoprovention of hepatocellular carcinorma with COX-2 selective inhibitors.
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| 关 键 词： | 环加氧酶-2 基因表达 肝细胞癌 肿瘤细胞 |
| 文章编号： | 1000-9604（2006）01-0032-06 |
| 收稿时间： | 2005-12-14 |
| 修稿时间： | 2006-06-09 |
Study on the expression of cyclooxygenase-2 in hepatocellular carcinoma cell lines and on the growth inhibition effect of NS-398 |
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| Kun Wang,Bao-cai Xing,Qing-yun Zhang,Guang-wei Xu. Study on the expression of cyclooxygenase-2 in hepatocellular carcinoma cell lines and on the growth inhibition effect of NS-398[J]. Chinese Journal of Cancer Research, 2006, 18(1): 32-37. DOI: 10.1007/s11670-006-0032-y |
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| Authors: | Kun Wang Bao-cai Xing Qing-yun Zhang Guang-wei Xu |
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| Affiliation: | (1) Department of Surgery, Peking University School of Oncology, Beijing Cancer Hospital, Beijing Institute for Cancer Research, Beijing, 10036, China |
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| Abstract: | Objective: To investigate the expression of cyclooxygenase -2 (COX-2) in hepatocellular carcinoma cell lines and to explore the effect of NS-398, a selective inhibitor for COX-2, on HepG-2 cell line. Methods: Immunohistochemistry and RT-PCR were used to investigate COX-2 expression in 6 HCC cell lines. MTT and Flowcytometry were used to evaluate the effect of the selective inhibitor of COX-2, NS-398, on HepG-2 cell lines. Results: All six HCC cell lines showed COX-2 expression at protein level. Five out of 6 cell lines showed COX-2 expression at mRNA level. NS-398 could suppress the growth of HepG-2 cell line, in a time and dose dependant manner. Conclusion: NS-398, a selective inhibitor of COX-2, showed inhibition effect on HepG-2 HCC cell line. The efficacy of inhibition was time and dose dependent, providing a new evidence for chemoprovention of hepatocellular carcinorma with COX-2 selective inhibitors. |
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| Keywords: | COX-2 inhibitor Hepatocellular carcinoma cell lines NS-398 |
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