离体人血受照后染色体培养中若干问题的探讨

探讨了人离体血经X线照射后，染色体培养过程中松胞素B（Cyt-B)及培养时间对分裂指数（MI）的影响。结果表明：（1）0-15Gy照射，培养体系中加入Gyt-B，MI均有所增加；10Gy以上影响较在，25Gy不再有影响。（2）6-25Gy，延长培养时间MI明显增加，在6-10G 间仅靠延长时间即可获足够分析用的分裂像。（3）加Cyt-B的同时延长培养时间，其MI高于单纯加Gyt-B或延长时间者，特别是15Gy，昭后只有加Gyt-B同时延长培养时间才能使MI增多至可供分析，以上结果提示：（1）辐射事故中当以染色体畸变估算大于6Gy受昭者的剂量时，必须延长培养时间，有条件者加Cyt-B方可增加培养成功的机会，提供足够的分裂像。（2）改变培养条件，加Cyt-B并延长培养时间有可能制定出剂量上限高于5Gy的剂量效应刻度曲线，从而进一步提高其作为生物剂量计的临床应用价值。

A STUDY OF SOME PROBLEMS IN CHROMOSOME CULTIVATION AFTER IONIZATION RADIATION OF HUMAN BLOOD IN VITRO

The effects of cytochalasin B (Cyt-B) and cultural time on mitotic index (MI) during chromosome culture of human peripheral blood irradiated by 6MV X-ray in vitro were studied. The results showed: (1) a successful cultivation with enough mitotic figures could be carried out in order to estimate the irradiation dose with chromosome aberrations and when the predicted dose was above 6 Gy in a radiation accident, when the predicted dose was up to 15 Gy the cultural time should be prolonged and Cyt-B should be added to the cultural medium; (2) it was possible to establish a dose effect calibration curve for doses above 5 Gy by adding Cyt-B and prolonging the cultural time, so that its value as a biological dosimeter for clinical application might be increased than before.