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Genomic cloning and partial characterization of human chymotrypsinogen gene |
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| Authors: | De-Xing Hou Kazuo Ozawa Naohiro Tomita Yoshizane Maeda Tsutomu Hashiguchi Kazushige Yokoyama Eiichi Soeda |
| Affiliation: | (1) Gene Bank, Tsukuba Life Science Center, The Institute of Physical and Chemical Research (RIKEN), 3-1-1 Koyadai, 305, Japan;(2) Department of Surgery II, Osaka University Medical School, Fukushima-ku, 553 Osaka, Japan;(3) Department of Animal Science, Faculty of Agriculture, Kagoshima University, 890 Kagoshima, Japan |
| Abstract: | Summary Chymotrypsinogen is a principal precursor of pancreatic proteolytic enzymes. We previously isolated a cDNA clone for human prechymotrypsinogen from a human pancreatic cDNA library. In the present study, we used this cDNA sequences to isolate genomic DNA clones. Three overlapping cosmid clones spanning approximately 65-kb genomic sequences were isolated from a human cosmid library. The genomic DNA clones were characterized by restriction enzyme mapping and by hybridizing them to subfragments of the cDNA. The sequence tagged sites for human chymotrypsinogen gene were created by designing two oligonucleotides. Furthermore, the isolated genomic clones were confirmed to be localized on chromosome 16q23 by fluorescencein situ hybridization and G-banding analysis. |
| Keywords: | chymotrypsinogen gene cosmid cloning genomic analysis chromosomal localization sequence tagged site |
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