海狗油脂肪乳对大鼠致畸毒性研究

背景与目的:探讨海狗油脂肪乳对大鼠致畸毒性作用。材料与方法:孕鼠设3个海狗油脂肪乳剂量组(800、400和200mg/kg)、环磷酰胺(CP)阳性对照组和溶剂对照组。在受孕大鼠妊娠的第6～15d连续尾静脉注射受试物10d,阳性对照组大鼠妊娠第10d一次性腹腔注射CP。结果:与溶剂对照组比较,海狗油脂肪乳200mg/kg剂量组的孕鼠体重增长缓慢(P<0.01);400mg/kg剂量组出现胎仔身长和体重减轻的胚胎毒性(P<0.05);800mg/kg剂量组出现吸收胎数增多(P<0.05)。各剂量组胎仔的黄体数、着床数、活胎数、死胎数、胎仔尾长和胎仔性别等与溶剂对照组间的差异无统计学意义(P>0.05)。海狗油脂肪乳各剂量组未见明显的骨骼畸形和内脏器官畸形。结论:海狗油脂肪乳剂量在200mg/kg时,对大鼠具有一定的母体毒性;海狗油脂肪乳在剂量400mg/kg时有胚胎毒性;在剂量800mg/kg时,未见大鼠胎仔骨骼和内脏器官的致畸毒性。

Teratogenicity of Seal Oil Emulsion in Rats

BACKGROUND AND AIM:To study the teratogenic effect of fat emulsion of seal oil in rats.MATERIALS AND METHODS:The Wistar rats were divided into three groups each trated with different dosages of test drug(800,400 and 200 mg/kg),one positive group(cyclophosphamide) and one negative control group(normal saline).On days 15-20 of pregnancy,the drug was injected via tail vein for ten days consecutively.RESULTS:The body weight gains of pregnant rats were significantly lower in the 200 mg/kg group than those of the control group(P<0.05).The embryonic body weights and body lengths of the 400 mg/kg group were significantly less than those of the control group(P<0.05).Compared with negative control group,the number of absorbed embryos was increased significantly in the 800 mg/kg group(P<0.05).The numbers of living embryos,absorbed embryos,dead embryos,the weight,body length and tail length showed no significant differences between the treated groups and the negative control group(P>0.05).No abnormaility was observed in the growth of the skeletal and internal organs in rat fetuses in any groups.CONCLUSION:Seal oil emulsion at 200 mg/kg caused maternal toxicity for pregnant rats,characterized by decreased body weight.Seal oil emulsion at 400 mg/kg led to embryotoxicity.Seal oil emulsion at 800 mg/kg had no teratogenic effects on skeletal and internal organs.