幽门螺杆菌感染和喉鳞状细胞癌致病因素的研究

目的 探讨幽门螺杆菌(Helicobacter pylori,Hp)在喉鳞状细胞癌患者中的感染情况,分析HP感染是否为喉鳞癌的致病因素.方法 采用巢式聚合酶链反应(nested PCR,nPCR)技术和Hp培养、鉴定对30例喉癌患者和15例喉良性疾病(声带息肉和会厌囊肿)患者的喉黏膜进行Hp检测.结果 采用nPCR法检测Hp,喉癌患者肿瘤组织Hp阳性22例(73.3%)高于对照组3例(20.0%),两者差异有统计学意义(x2=11.520,P=0.010).在22例Hp(+)的喉癌患者的44份肿瘤和癌旁组织中,有19份癌旁正常组织检出Hp(86.4%),而10份肿瘤组织检出Hp(45.4%),两者差异有统计学意义(x2=4.697,P=0.030).所有标本Hp培养均为阴性.结论 Hp在喉癌患者中的感染率明显高于实验人群,可能是喉癌的致病因素之一.

Helicobacter pylori infection: a potential pathogenic factor for laryngeal squamous cell carcinoma

Objective To investigate the difference in Helicobacter pylori (Hp) infection rate between the patients with laryngeal squamous cell carcinoma and the patients with benign laryngeal lesions and to explore the role of Hp infection in the pathogenesis of laryngeal squamous carcinoma. Methods Nested polymerase chain reaction(nPCR) and Hp culture were used to identify the Hp in laryngeal mucosa in 30 patients with laryngeal carcinoma and 15 patients with benign laryngeal lesions including polyps of vocal cords or epiglottic cysts. Results nPCR showed that Hp-positive rate (73.3%) of patients with laryngeal carcinoma was significantly higher than that( 20.0% )of control patients with benign laryngeal lesions (x2 =11.520,P =0.010). Regarding the 22 positive neoplastic cases that have 44 tissues, out of the 22 tumor tissues,10 were positive with Hp (45.4%), and among 22 tissues beside the tumor,19 were positive(86.4% ). This event also indicated a statistical significance ( x2 = 4.697, P = 0.030 ). Hp culture showed that Hp was negtive in all specimens. Conclusions Hp exist in the laryngeal mucosa, but with the higher rate of Hp infection in the patients with laryngeal squamous cell carcinoma than that in the patients with benign laryngeal lesions, which suggests Hp may be one of pathogenic factors of laryngeal squamous cell carcinoma.