| 结核分枝杆菌稳定L型变异的聚合酶链反应研究 |
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| 引用本文： | 王豫萍,王和. 结核分枝杆菌稳定L型变异的聚合酶链反应研究[J]. 贵阳医学院学报, 2004, 29(5): 389-391 |
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| 作者姓名： | 王豫萍 王和 |
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| 作者单位： | 贵阳医学院,微生物学教研室,贵州,贵阳,550004 |
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| 基金项目： | 贵州省科技厅资助项目 (黔基合计字 2 0 0 1 30 885) |
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| 摘 要： | 目的：探讨结核分枝杆菌稳定L型变异的分子机制。方法：采用结核分枝杆菌聚合酶链反应(PCR)诊断试剂扩增结核分枝杆菌稳定L型纯培养物的染色体DNA，琼脂糖凝胶电泳、聚丙烯酰胺凝胶电泳(polyscrylamid gel electrophoresis，PAGE)薄层凝胶扫描分析。结果：结核分枝杆菌稳定L型比细菌型多一DNA条带。结论：结核分枝杆菌稳定L型的形成可能与基因突变有关，在特异性PCR反应中可形成与其亲代细菌型有差异的产物。
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| 关 键 词： | 分枝杆菌 结核 L型菌 聚合酶链反应 |
| 文章编号： | 1000-2707(2004)05-0389-03 |
| 修稿时间： | 2003-04-03 |
A Study on Variation of Stable L-form of Mycobacterium tuberculosis with PCR |
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| WANG Yu-ping,WANG He. A Study on Variation of Stable L-form of Mycobacterium tuberculosis with PCR[J]. Journal of Guiyang Medical College, 2004, 29(5): 389-391 |
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| Authors: | WANG Yu-ping WANG He |
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| Abstract: | Objective: To probe the molecular mechanism of the variation of stable L-form of Mycobacterium tuberculosis.Methods: The chromosome DNA of stable L-form of M. tuberculosis was analysed with polymerase chain reaction (PCR), agarose gel electrophoresis (AGE), polyacrylamid gel electrophoresis (PAGE) and Tcl scanner.Results: Two electrophoresis bands of DNA were found in the stable L-form. One was similar to that of parental bacteria and the other one was smaller.Conclusion: Mutation of chromosome gene would happen in the stable L-form of M. tuberculosis and a new DNA band might occur. |
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| Keywords: | Mycobacterium tuberculosis L forms polymerase chain reaction |
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