| 鼻咽癌细胞中增强型表达载体调控TK基因与端粒酶活性关系的研究 |
| |
| 引用本文： | 申聪香,文忠,钱宇虹,牟少凤,关小芳.鼻咽癌细胞中增强型表达载体调控TK基因与端粒酶活性关系的研究[J].临床耳鼻咽喉头颈外科杂志,2010,24(4). |
| |
| 作者姓名： | 申聪香 文忠 钱宇虹 牟少凤 关小芳 |
| |
| 作者单位： | 南方医科大学珠江医院耳鼻咽喉-头颈外科,广州,510282 |
| |
| 基金项目： | 广东省科技计划项目资助(No:2007B031003008) |
| |
| 摘 要： | 目的:探讨人端粒酶催化亚单位(hTERT)基因核心启动子和原核增强子CMV联合调控单纯疱疹病毒胸苷激酶基因/更昔洛韦系统对TK基因的活性改变及与鼻咽癌细胞中端粒酶活性的关系。方法:将改良构建后的增强型载体pGL3-basic-EGFP-TK-hTRETp-CMV enhancer及单启动子载体pGL3-basic-EGFP-TK-hTRETp(作为对照组)分别转染端粒酶阳性的人鼻咽癌5-8F细胞株及对照组细胞人乳腺癌MCF-7细胞(端粒酶阳性)及正常人血管内皮ECV细胞(端粒酶阴性),采用荧光显微镜下观察其TK基因绿色荧光蛋白表达,实时荧光定量PCR方法检测转染细胞中TK基因mRNA定量表达差异,TRAP银染法检测肿瘤细胞转染前后端粒酶活性改变并分析TK基因表达与端粒酶活性之间的关系。结果:①增强型表达载体转染鼻咽癌5-8F细胞及乳腺癌MCF-7细胞均有很强的荧光表达及TK基因的mRNA表达,比单启动子pGL3-basic-EGFP-TK-hTRETp及ECV细胞绿色荧光要强。实时荧光定量PCR显示,增强型载体组A值亦较对照组明显增高。②转染增强型载体(加GCV)后TRAP银染法检测鼻咽癌5-8F细胞端粒酶活...
|
| 关 键 词： | 鼻咽肿瘤 增强型表达载体 TK基因 端粒酶 |
Study on the relationship between TK gene regulated by enhanced suicide gene vector and telomerase activity in nasopharyngeal carcinoma cells |
| |
| SHEN Congxiang,WEN Zhong,QIAN Yuhong,MU Shaofeng,GUAN Xiaofang.Study on the relationship between TK gene regulated by enhanced suicide gene vector and telomerase activity in nasopharyngeal carcinoma cells[J].Journal of Clinical Otorhinolaryngology,2010,24(4). |
| |
| Authors: | SHEN Congxiang WEN Zhong QIAN Yuhong MU Shaofeng GUAN Xiaofang |
| |
| Institution: | Department of Otolaryngology-Head and Neck Surgery/a>;Pearl River Hospital/a>;Nanfang Medical University/a>;Guangzhou/a>;510282/a>;China |
| |
| Abstract: | Objective:To explore the relationship between TK gene expression regulated by enhanced suicide gene vector and telomerase activity in nasopharyngeal carcinoma cells.Method:The reformed reconstructed enhanced vector,pGL3-basic-EGFP-TK-hTRETp-CMV enhancer,and hTERT mono-promoter vector,pGL3-basic-EGFP-TK-hTRETp(as controls),were transfected into telomerase(+) nasopharyngeal carcinoma 5-8F cell lines,telomerase(+) human breast cancer MCF-7 cell lines and telomerase(-) normal vascular endothelium cell lines res... |
| |
| Keywords: | nasopharyngeal neoplasms enhanced suicide gene vector TK gene telomerase |
| 本文献已被 CNKI 万方数据 等数据库收录！ |
