长链非编码RNA NR2F1-AS1靶向微小RNA-145-5p调控结肠癌细胞增殖、迁移和侵袭的分子机制

目的 探讨长链非编码RNA(lnc RNA)核受体亚族2F组成员1反义RNA(NR2F1-AS1)对结肠癌细胞增殖、迁移和侵袭的影响及作用机制.方法 收集2015年3月至2017年5月青海省第五人民医院外科手术切除并经病理证实为原发性结肠腺癌组织25例,另留取距肿瘤边缘3 cm的癌旁组织(正常结肠组织).实时荧光定量P...

Molecular mechanism of lnc RNA NR2F1-AS1 targeting miR-145-5p to regulate colon cancer cell proliferation, migration and invasion

Objective To investigate the effect and mechanism of lnc RNA NR2F1-AS1 on the proliferation, migration and invasion of colon cancer cells.Methods From March 2015 to May 2017, 25 patients with primary colorectal adenocarcinoma confirmed by pa.thology and surgically resected in The Fifth People''s Hospital of Qinghai Province were collected. Paracancental tissue (normal colontissue) 3 cm away from the tumor edge was also collected. The expression levels of NR2F1-AS1 and miR-145-5p in colon cancer tissues and adjacent tissues were detected by RT-qPCR. The colon cancer cell HCT116 was used as the research object, and the double lucifer.ase reporter gene experiment verified the regulatory relationship between NR2F1-AS1 and miR-145-5p. NR2F1-AS1 small interfering RNA or miR-145-5p mimic was transfected into HCT116 cells. CCK-8 and Transwell were used to detect the effect of interfering with NR2F1-AS1 expression or over-expressing miR-145-5p on the proliferation, migration and invasion of HCT116 cells.Results Com. pared with adjacent tissues, the expression of NR2F1-AS1 in colon cancer tissues increased (3.39±0.33) vs. (1.01±0.11), P<0.05], and the expression of miR-145-5p decreased(0.55±0.05) vs. (1.00±0.08), P<0.05]. Compared with the miR-NC group co-transfected with WT-NR2F1-AS1, the luciferase activity in miR-145-5p group was reduced (P<0.05). After over-expressing NR2F1-AS1, the expression level of miR-145-5p in cells decreased (P<0.05), and the expression level of miR-145-5p in cells increased after interfering with sNR2F1-AS1 expression (P<0.05). After interfering with sNR2F1-AS1 expression or over-expressing miR-145-5p, the OD value, HCT116 cells migration and invasion numbers were decreased (P<0.05). Interfering with miR-145-5p expression reversed the effect of interfering with NR2F1-AS1 expression on HCT116 cells OD value, migration and invasion numbers.Conclusion NR2F1-AS1 is highly expressed in colon cancer tissues. Interfering with NR2F1-AS1 expression may inhibit colon cancer cell proliferation, migration and invasion by up-regulating miR-145-5p expression, and is a potential target for colon cancer treatment.