微小RNA-20a、微小RNA-92a在乳腺癌诊断及预后评估中的应用

目的 分析微小RNA(miR)-20a、miR-92a在乳腺癌病人血清中的表达情况,并探讨其对乳腺癌诊断及预后评估的价值.方法 选取2012年1月至2013年5月中国人民解放军中部战区总医院首次确诊为乳腺癌并经手术治疗的病人89例为观察组,以80例同期健康体检者为健康对照组,采用实时荧光定量逆转录聚合酶链式反应(qRT...

Application of microRNA-20a and microRNA-92a in the evaluation of breast cancer diagnosis and prognosis

Objective To analyze the expression of microRNA-20a and microRNA-92a in serum of patients with breast cancer, and to explore their value in diagnosis and prognosis evaluation of breast cancer.Methods Eighty-nine patients with breast cancer whowere diagnosed for the first time and treated surgically in Central Theater General Hospital of PLA from January 2012 to May 2013were selected as the study subjects, and 80 healthy people in the same period were taken as the control group, the levels of serum miR-20a and miR-92a in patients with breast cancer before and after operation and in control group were detected by qRT-PCR, the relation. ships between the expressions of miR-20a and miR-92a with clinicopathological features were analyzed. Spearman correlation analysiswas used to analyze the correlation between the expressions of serum miR-20a and miR-92a, ROC curve was used to analyze the diag. nostic values of miR-20a, miR-92a, and their combined detection in breast cancer, Kaplan-Meier method was used to analyze the ef. fects of the expressions of miR-20a and miR-92a on patients'' 5-year disease-free survival (OS), Logistic regression analysis was used to analyze the prognostic factors of breast cancer.Results The levels of serum miR-20a [before operation (1.76±0.15), after operation (1.38±0.12) vs. (1.02±0.08)] and miR-92a [before operation (1.83±0.13), after operation (1.45±0.11) vs. (1.11±0.07)] in breast cancer pa. tients were significantly higher than those in control group (P<0.05). The expressions of miR-20a and miR-92a were correlated with tu. mor differentiation, TNM stage, and lymph node metastasis (P<0.05). There was a positive correlation between the expressions of serum miR-20a and miR-92a in breast cancer patients (r=0.524, P<0.05). The sensitivity, specificity, and AUC of the combination of miR-20a and miR-92a in the diagnosis of breast cancer were significantly increased (P<0.05). The 5-year OS of patients with low expressions of miR-20a and miR-92a was significantly higher than that of patients with high expression of miR-92a [miR-20a (93.24% vs. 73.28%), miR-92a (94.85% vs. 74.57%), P<0.05]. miR-20a and miR-92a, differentiation degree and lymph node metastasis were independent risk factors affecting the prognosis of breast cancer patients (P<0.05).Conclusion The expressions of serum miR-20a and miR-92a are abnormal in breast cancer patients, they are related to the degree of differentiation of tumors, TNM stage, and lymph node metasta.sis, which may be used in the diagnosis and prognosis of breast cancer.