妊娠期合并心脏病病人血清中MTHFR基因C677T位点多态性与母婴结局关系

目的 探讨妊娠期合并心脏病病人血清中MTHFR基因C677T位点多态性与母婴结局关系.方法 选取2018年2月至2019年10月德州市妇女儿童医院就诊的妊娠期合并心脏病病人88例(妊娠期心脏病组)及同期健康孕妇92例(对照组),通过聚合酶链式反应-限制性片段长度多态性(PCR-RELP)法检测受试者血清MTHFR基因C...

Relationship between C677T polymorphism of MTHFR gene and maternal and infant out. comes in pregnant women with heart disease

Objective To investigate the relationship between C677T polymorphism of MTHFR gene in serum and maternal and in.fant outcomes in pregnant women with heart disease.Methods 88 pregnant women with heart disease during pregnancy and 92healthy pregnant women in the same period were selected as the study objects in Dezhou Women''s and Children''s Hospital from Febru.ary 2018 to October 2019, the distribution frequency of C677T locus on MTHFR gene was detected by polymerase chain reaction re.striction fragment length polymorphism (PCR-RELP), and the relationship between MTHFR single nucleotide genotypes and pregnancy outcomes was analyzed.Results The genotypes of C677T locus on MTHFR gene included CC wild type, CT heterozygote and TT ho.mozygote; the genotypes of C677T locus on MTHFR gene in the control group and the heart disease during pregnancy group conformedto Hardy-Weinberg equilibrium law; compared with the control group, the frequencies of CT, TT genotypes and T allele in C677T locusof MTHFR gene in heart disease during pregnancy group were significantly increased (P<0.05); the incidences of fetal macrosomia, neo.natal hypoglycemia, asphyxia neonatorum and premature in pregnant women of CT type and TT type in heart disease during pregnancygroup were higher than those in CC group; the incidences of fetal macrosomia, neonatal hypoglycemia, asphyxia neonatorum and prema.ture in pregnant women of TT type were higher than those in heterozygote CT type (P<0.05); and binary logistic regression analysisshowed that CT heterozygote and TT homozygote mutation at C677T locus on MTHFR gene were risk factors for adverse pregnancy out.come in patients with heart disease during pregnancy (P < 0.05).Conclusion The C677T polymorphism of MTHFR gene in serum isclosely related to the maternal and infant outcomes of heart disease during pregnancy.