角膜移植后患者角膜新生淋巴管与新生血管和炎症的关联

目的:研究角膜移植后角膜新生淋巴管与新生血管和炎症的关联。方法:人角膜取自行二次角膜移植的患者19例。淋巴内皮细胞受体(lymphatic vessel endothelial hyaluronan receptor,LYVE-1)和内皮细胞黏附因子-1(platelet endothelial celladhesion modecule-1,PECAM-1)双重免疫组化法标记角膜中的新生血管和淋巴管,进行淋巴管计数(lymphatic ves-sels counting,LVC)和血管计数(blood vessels counting,BVC),比较BVC、炎症指数(inflammation index,IF)、移植历史(transplantation history,TH)与LVC之间的关联。结果:角膜移植后BVC,IF与LVC间均呈显著性正相关,而TH与LVC间呈显著性负相关。角膜移植后新生淋巴管、血管、眼表炎症间大致成平行发展,新生淋巴管最先退化,其次是眼表炎症,新生血管最后消退。结论:人角膜移植后角膜新生淋巴管与新生血管、眼表炎症之间存在着极为密切的关联。

Clinical research of corneal lymphangiogenesis after corneal transplantation

·AIM:To discuss the association of corneal hemangio-genesis,corneal inflammation with corneal lymphangio-genesis after human corneal transplantation.·METHODS:Nineteen human corneas were obtained from 19 patients undergoing a second corneal transplan-tation from 2005 to 2008.Corneal hemangiogenesis and lymphangiogenesis were examined by lymphatic vessel endothelial hyaluroman receptor(LYVE-1)and platelet endothelial cell adhesion modecule-1(PECAM-1)double immunohisto-chemistry.The state of corneal inflamma-tion was evaluat-ed by inflammation index(IF)grading.Then,the association of blood vessel counting(BVC),IF,corneal transplantation history(TH)with lymphatic vessel count-ing(LVC)was examined.·RESULTS:LVC was strongly and positively correlated with both BVC(r=0.725;P<0.01)and IF(r=0.661;P<0.01)in transplanted human corneas.However,LVC was significantly and inversely correlated with TH(r=-0.301;P<0.01).Although the development of corneal lymphangio-genesis,hemangiogenesis and inflammation was parallel in general,corneal lymphangiogenesis disappeared followed by the inflammation of corneas and corneal blood vessels.·CONCLUSION:Corneal lymphangiogenesis correlates closely with corneal hemangiogenesis and inflammation after human corneal transplantation.·