黄连解毒汤中1个新的黄酮苷

目的:研究黄连解毒汤(由黄连、黄芩、黄柏、栀子等4味中药组成的复方)的化学成分,为其药代动力学和药效动力学研究提供物质基础.方法:采用各种柱色谱方法进行分离、纯化,NMR和MS等方法进行结构鉴定.结果:从黄连解毒汤的醋酸乙酯可溶性部分得到1个新的黄酮苷,命名为汉黄芩素-5-O-β-D葡萄糖醛酸苷甲酯(wogonin-5-O-β-D-glucuronide methyl ester,1).从正丁醇可溶性部分得到10个化合物,分别鉴定为小檗碱(2),巴马汀(3),表小檗碱(4),京尼平苷(5),药根碱(6),非洲防己碱(7),格陵兰黄连碱(8),汉黄芩苷(9),3,5-二乙酰胺吡啶(10)和京尼平-1-O-β-D-龙胆双糖苷(11).结论:化合物1为新化合物.根据已报道4味中药化学成分的研究结果,判断化合物2,3和6来源于黄柏和黄连;化合物4,7和8来源于黄连;化合物5和11来源于栀子;2个黄酮苷来源于黄芩.

A new flavonoid glucoside from Huanglianjiedutang decoction

Objective: To study the constituents from the water extractive of Huanglianjiedutang, which is composed of Rhizoma Coptidis, Radix Scutellariae, Cortex Phellodendri and Fructus Gardeniae,and provide substances foundation for its pharmacokinetic and pharmacodynamic investigation.  Method: The chemical constituents were isolated by various column chromatographic methods and structurally elucidated by NMR and MS techniques.  Result: A flavonoid glucoside identified as wogonin-5-O-β-D-glucuronide methyl ester (1) was isolated from the ethyl acetate soluble parts in the water extractive of Huanglianjiedutang decoction. Ten compounds were isolated from the butanol soluble parts in the water extractive of Huanglianjiedutang decoction and have been identified as berberine (2), palmatine (3), epiberberine (4), geniposide (5), jatrorrhizine (6), columbamine (7), groenlandicine (tetradehydrocheilanthifoline, 8), wogonoside (9), 3,5-pyridinedicarboxamide (10), and genipin-1-O-β-D-gentiobioside (11).  Conclusion: Compound 1 was a new flavonoid glucoside. On the basis of reported results of the chemical constituents of Rhizoma Coptidis, Radix Scutellariae, Cortex Phellodendri and Fructus Gardeniae, it was estimated that berberine,palmatine and jatrorrhizine rised from the Cortex Phellodendri and Rhizoma Coptidis; epiberberine,columbamine and groenlandicine rised from the Rhizoma Coptidis; geniposide and genipin-1-O-β-D-gentiobioside rised from the Fructus Gardeniae; wogonin-5-O-β-D-glucuronide methyl ester and wogonoside rised from the Radix Scutellariae.