系统性红斑狼疮患者血清相关自身抗体检测的临床意义

目的 探讨抗核小体抗体（anti-nucleosome antiboay，ANuA）、抗核糖体P蛋白抗体（anti-ribosomal P protein antiboay，ARPA）在系统性红斑狼疮（SLE）中的临床意义，了解其与SLE病情的关系。方法 分别用酶联免疫吸附（ELISA）法和欧蒙酶免疫斑点法检测SLE患者及疾病对照组患者血清中的ANuA及核糖体P蛋白抗体。分析它们之间的关系。结果 90例SLE患者中64例血清ANuA阳性，阳性率为71．1％，AR．PA阳性23例。阳性率25．6％，疾病对照组仅6．7％及4．4％，SLE患者ANuA、抗核糖体P蛋白抗体显著高于疾病对照组。结论 ANuA、ARPA是SLE诊断的重要的血清学指标，其中ANuA与SLE疾病活动性密切相关；ARPA与神经系统损伤和关节炎的发病有关，联合检测能对SLE的诊断、治疗提供重要依据。

Study on the relationship between autoantibodies and systemic lupus erythematosus

Objective To investigate the clinical sinificance of determing anti-nucleosome antibody and anti-ribosomal p protein antibody in SLE and to analysis the relationship in SLE. Methods The enzyme-linked immunosorbent assay (ELISA) and enzyme-immuno-Blot method was used to measure the ANuA and ARPA in serum of 90 SLE patients, 45 patients with other rheumatic diseases. Results Of the 90 SLE patients, 71.1% were seropositive for ANuA, 25.6% were seropositive for ARPA. Only 6.7% and 4.4% control patients were seropositive. The positive rate of ANuA and ARPA in SLE was obviously higher than patients with other diseases. Conclusions ANuA and ARPA are important serum markers in the diagnosis of SLE. They have a close relationship with the activity of SLE. Forthermore, ARPA has a close relationship of neurological lesions and arthritis. The detections of ANuA and ARPA with other auto-antibodies together is of great significance.