固相萃取-高效液相色谱法测定紫杉醇脂质纳米粒体外释放度

目的:建立测定紫杉醇脂质纳米粒体外释放度的方法,考察紫杉醇脂质纳米粒24h的体外释放度。方法:采用固相萃取-高效液相色谱法。样品经C18固相萃取柱萃取,采用Phenomenex luna C18(250mm×4.6mm,5μm)色谱柱分离,流动相为甲醇-水(80:20),流速为1.0mL.min-1,检测波长为227nm。结果:紫杉醇的检测浓度在2～12μg.mL-1范围内与峰面积积分值呈良好线性关系(r=0.9993),紫杉醇脂质纳米粒在水杨酸钠(1mol.L-1)-PBS(pH7.4)中24h累积释放度为104.9%,其释放规律符合一级动力方程。结论:所建方法简便、灵敏,干扰小,可用于体外释放的检测。

Determination of in Vitro Drug Release of Paclitaxel Lipid Nanoparticles by Solid Phase Extraction-HPLC

OBJECTIVE:To develop a solid phase extraction(SPE)-HPLC method for in vitro drug release of Paclitaxel lipid nanoparticles,and to investigate in vitro drug release of Paclitaxel lipid nanoparticles within 24 h.METHODS:The sample was purified with C18 solid phase extraction columns.The chromatographic analysis was performed on a phenomenex luna C18(250 mm×4.6 mm,5 μm) column with mobile phase consisted of methanol-water(80:20) at the flow rate of 1.0 mL·min-1.The detection wavelength was set at 227 nm.RESULTS:The linear range of paclitaxel was 2～12 μg·mL-1(r=0.999 3).The in vitro cumulative release of paclitaxel in 1 mol·L-1 sodium salicylate solution-PH 7.4 PBS reached about 104.9% in 24 h.The profile was described by first-class equation.CONCLUSION:The method is simple,sensitive and little interference,and it is suitable for the determination of in vitro drug release of sample.