皮肤撕脱伤撕脱皮瓣中内皮素生成及分布的意义

探讨内皮素的生成与皮肤撕脱伤后撕脱皮瓣继发坏死的关系。方法：采用家猪下肢上肤撕脱伤模型。应用免疫组织化学和图像分析法观察脱皮瓣组织中ＥＴ的分布，用放射免疫法测定血浆和撕脱皮瓣组织中ＥＴ的含量。结论：ＥＴ合成增多有是导致撕脱皮瓣继发坏死的重要原因之一。

Production and distribution of endothelin in the avulsed skin flaps

Objective: To study the relationship between the production of endothelin (ET) and the secondary necrosis of the avulsed skin flaps. Methods: The avulsed skin flap model of the domestic pig's legs was manufactured with a special designed imitating machine. Immunocytochemistry and auto imagine analysis were employed to observe the distribution of ET in the skin flap tissue and radioimmunoassay to measure the content of ET in plasma and the avulsed skin flaps. Results: ET was largely distributed in the endothelial layer of skin micro vessels and the production of ET increased with the necrosis of the avulsed skin flaps. In comparison with the control group, the content of ET in the experimental group was significantly increased, up to five times in the plasma and sixteen times in the avulsed skin flaps at 72 hours after injury. The two group values showed positive correlation ( r =0.87, P <0.01). Conclusion: The increase of ET content might be one of the causes leading to the secondary necrosis of the avulsed skin flaps.