口腔常见链球菌代谢组学鉴定的研究

目的通过比较血链球菌和远缘链球菌的代谢物图谱，初步将基于核磁共振的代谢组学方法应用于口腔常见链球菌的快速鉴定。方法在液体TPY培养基中分别接种相同密度的血链球菌和远缘链球菌菌悬液，用比浊法测定菌悬液密度，绘制生长曲线。取2种细菌生长稳定期的培养液检测核磁共振图谱，进行主成分分析。结果主成分分析表明2种细菌的数据内部具有集中的聚类关系，该方法可以区分这2种细菌。结论代谢组学方法在口腔链球菌的快速鉴定中具有良好的应用前景。

Discrimination of common oral streptococcus with metabonomics method

Objective To evaluate the feasibility of identifying oral streptococcus by comparing their metabolic profiling, and to find a convenient and rapid way to discriminate oral microorganisms. Methods The pure cultivation of Streptococcus sanguis ATCC 10556 and Streptococcus sobrinus 6715（reference strain） from solid culture were respectively inoculated in TPY liquid medium. Then the growth quantity was measured periodically by turbidimetry and the growth curves of the inoculated bacteria were completed. The culture solutions in the stationary phase of the two bacteria were centrifuged, and then tested with the 1H-nuclear magnetic resonance（1H-NMR） spectrometer respectively. The gained free induction decay（FID） data were all inputted into MestReC Soft and finally transformed into metabolic profiling. The metabolic profils were integrated segmentingly and the results were inputted into SIMCA-P Soft for principal components analysis（PCA）. Results The PCA results showed the obvious clustering phenomena and the points of two group data differentially centralized in two clusters. Therefore, the NMR-based metabonomics profiles can discriminate the two different kinds of bacteria. Conclusion The metabonomics can be expected to be a kind of promising useful method in quick discrimination of oral streptococcus.