内毒素血症在肝癌发生发展中的作用

目的:探讨内毒素血症在肝癌发生发展中作用。方法:利用饮水中加入0.03%TAA,4个月形成肝硬化,6个月形成肝癌动物的模型。TAA+LPS组从第5个月开始皮下注射内毒素,至实验结束。在肝癌发生发展过程中进行血浆内毒素水平、γ-GT活性、DNA指数、增殖指数,并对N-ras、p53基因点突变进行分析。结果:内毒素可增加bcl-2与p53蛋白过度表达与增加N-ras、p53基因的点突变,增加自由基生成与降低抗氧化酶活性,加重DNA损伤。结论:内毒素能够促进TAA诱发肝癌变的过程。

Role of endotoxemia in the development of hepatocarcinoma in rats

AIM: To explore the effect of endotoxemia on hepatocarcinoma course induced by TAA. METHODS: The rat cirrhosis was induced by intaking TAA (0.03%) for four months, hepatocarcinom for six months. Lipoplysaccharide (100 μg/kg) was administered subcutaneously every two days from the fifth month to the end of sixth month in TAA-treated rats. The plasma endotoxin content, γ-glutamyl transpeptidase(γ-GT) activity, DNA index and aneuploidy index were determined, the point mutation of N-ras, p53 gene was detected in hepatocarcinoma rats. RESULTS: Endotoxin increased protein overexpression of p53, bcl-2 and N-ras , p53 gene mutation point and free radical production, reduced antioxidase activity and aggravated DNA damage in hepatocarcinoma rats. CONCLUSION: Endotoxin could accelerate hepatocarcinogenesis promoted by TAA.