Characterization of variable-region genes and shared crossreactive idiotypes of antibodies specific for antigens of various influenza viruses |
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| Authors: | T M Moran M Monestier A C Lai G Norton M A Reale M A Thompson J L Schulman R Riblet C A Bona |
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| Institution: | Department of Microbiology, Mount Sinai School of Medicine, New York, NY 10029. |
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| Abstract: | Several syngeneic monoclonal anti-idiotypic antibodies were obtained against PY206, a monoclonal antibody specific for X-31 (H3N2) influenza virus hemagglutinin. This idiotype was found in the sera of BALB/c mice immunized with various influenza viruses. Adsorption experiments indicated that the PY206 Id was borne by antibodies specific for viral hemagglutinin (HA) and/or neuraminidase (NA). This idiotype was identified on other monoclonal antibodies specific for various influenza HAs (H3 and H1). Study of the variable-region (V) genes of these monoclonal antibodies showed that its expression is independent of variable kappa (VK)21 light-chains and that the heavy-chains of the strongly idiotype-positive hybridomas derive from either the variable heavy (VH) J558 or VH 7183 family. Finally, Western blot analysis demonstrated that PY206 idiotypic determinants are located exclusively on the heavy chain. |
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