| 三磷酸腺苷生物荧光液闪测定法检测活细胞计数 |
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| 引用本文： | 刘珊玲,彭芝兰,王和,楼江燕,何斌,唐茜萍,邱东升.三磷酸腺苷生物荧光液闪测定法检测活细胞计数[J].四川大学学报(医学版),2000,31(2):262-263,268. |
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| 作者姓名： | 刘珊玲 彭芝兰 王和 楼江燕 何斌 唐茜萍 邱东升 |
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| 作者单位： | 华西医科大学附属第二医院,妇产科,成都,610041 |
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| 基金项目： | 高等学校博士学科点专项科研项目，， |
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| 摘 要： | 为建立一种检测活细胞数的方法,作者采用三磷酸腺苷(ATP)生物荧光液闪测定法检测小鼠纤维瘤细胞株L929.ATP标准曲线工作范围为10 -9～10-5mol/ml,相关系数r=0.9963(P＜0.001);当活细胞数在3×1 02～106个/ml时与发光计数值有良好的线性关系,r=0.9922(P＜0.001),变异系数 CV = 1%～3%.用ATP生物荧光液闪测定法检测活细胞数灵敏、简便、准确、重复性好,用液闪计数仪即可完成测定,有较大的实用价值.
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| 关 键 词： | 三磷酸腺苷 生物荧光液闪测定 细胞计数 |
An Adenosine Triphosphate Bioluminescence Assay for Detecting the number of Living Cells |
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| Liu Shanling,Peng Zhilan,Wang He,Lou Jiangyan,He Bin,Tang Qianping,Qiu Dongsheng.An Adenosine Triphosphate Bioluminescence Assay for Detecting the number of Living Cells[J].Journal of West China University of Medical Sciences,2000,31(2):262-263,268. |
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| Authors: | Liu Shanling Peng Zhilan Wang He Lou Jiangyan He Bin Tang Qianping Qiu Dongsheng |
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| Abstract: | The rnethod for detecting the number of living cells was studied. Using an adenosine triphosphate (ATP) bioluminescence assay, the present authors reported a perfect linear relationship between lg ATP concentrations and lg luminescence counts (r=0. 9963) as well as a relationship between lg number of cells and lg ATP luminescence counts (r=0. 9922). The detectable cells ranged from 102 to 106 cells/ml, the coefficients of variation 1-3%. This method is simple, accurate and sensitive and has a high reproducibility. |
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| Keywords: | Adenosine triphosphate Bioluminescence assay Cell count |
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