用分子生物学技术分析国内六株旋毛虫分离株

本文首次对来自我国不同地区、不同宿主来源有代表性的6个旋毛虫分离株从基因组DNA的限制性酸切长度多态性、同工酶及可溶性蛋白进行综合研究。核酸结果显示：长春株与其余各地域株的限制性酶切困话间存在着差异，用长春株特异性DNA片段（1．12kb）制成探针，与各株DNA进行Southern杂交，发现各虫株的杂交带型不一，且仅长春株出现1．12kb杂交带。同工酶结果显示：长春株与其余各珠在5种同工酶（GPI、G6PD、HK、6PGDH及AK）酶谱中有显著不同。等电聚焦电泳结果显示：长春株具有一条约4.1PI的特异带。上述结果提示：我国6株旋毛虫分离株间存在着差异，长春株与其余各株差异显著，从而推断我国旅毛虫虫株至少存在2种不同的生物学类型，其间的差异主要与不同的地理分布和／或不同宿主来源有关。

ANALYSIS OF CHINESE ISOLATES OF TRICHNELLA SPIRALIS BY MOLECULAR BIOTECHNOLOGY

To investigate the relationships among Trichinella spiralis isolates obtained from different geographical regions and hosts in China, six isolates of T. spiralis obtained from Changchun, Tianjin, Xi'an, Henan and Yunnan were detected. All of the isolates were secured from pigs except the Changchun isolate which was from a dog. Restriction fragment length polymorphism (RFLP), isoenzyme electrophoresis and soluble protein analysis were used. Distinctive banding patterns in restriction maps were seen between Changchun and other isolates studied. A cloned specific repetitive DNA sequence (1. 12kb) from Changchun isolate was then select-ed to prepare probe for Southern blotting of DNA fragments. The hybridizing banding patterns among the iso-lates were different and only the Changchun isolate showed the 1.12kb band. The Changchun isolate was also distinguishable from the other isolates in isoenzyme patterns of GPI,G6PD,HK, 6PGDH and AK,as well as in isoelectric focusing electrophoresis where the Changchun isolate presented a specific band at 4.1 PI. There areat least two different biologica1 types of T. spiralis in China and the differences between them may be attribut-ed to different geographical origins and/or different hosts.