血小板源生长因子在低氧性肺动脉平滑肌细胞增殖中的作用

运用流式细胞仪及细胞原位杂交方法测定低氧对培养的新生牛肺动脉平滑肌细胞（ＰＡＳＭＣ）生长的影响，并探讨血小板源生长因子（ＰＤＧＦ）在其中的作用，结果表明：培养的ＰＡＳＭＣ的低氧１２ｈ时，由Ｇ０／Ｇ１期进入Ｓ斯的细胞数明显增多，为对照组的１５７．７％（Ｐ〈０．０５）；ＰＤＧＦ可进一步促进低氧状态下ＰＡＳＭＣ的增殖，Ｓ期细胞为对照组的２４２．３％（Ｐ〈０．０１），原位杂交结果显示：低氧（１２ｈ）条件下

Effect of Platelet-derived Growth Factor on Proliferation ofHypoxic Pulmonary Artery Smooth Muscle cell

By means of flow cytometry and in situ hybridization ,we investigated the role of hypoxia in cultured neonatal bovine pu,lmonary artery smooth muscle cells (PASMC ) ,and the effect of PDGF on that role. Cell-cycle analysis revealed hypoxia ( 12h ) and PDGF added meantime had effect on the G/G S transition , both could increase the number of cells entering S phase from G/G The percents of S phase cells reached to 41%and 63%respectivly , and werel 57. 7% and 2.2. 3% compared with normoxiit (P<0. 05). Using in situ hybridization , we detected that hypoxia (12h ) upregulated PDGF-A,B chain and receptor gene expres- sion (all P<0. 001). Results therefore suggested that increase of PDGF-A ,B chain and receptor gene elxpression under hypoxia condition and effect of PDGF cooperated with hypox- ia on PASMCs proliferation may contribute to the pulmonary vascular remodeling.