基于UFLC-Q-TOF/MS分析黄芪-丹参药对化学成分研究

目的?应用UFLC-Q-TOF/MS对黄芪-丹参药对中的化学成分进行鉴定。方法?采用Thermo BDS C₁₈(3μm，150mm×2.1mm)色谱柱，以0.1%甲酸水-0.1%乙腈为流动相进行梯度洗脱，流速为0.3mL/min，柱温40℃，进样量5μL。离子源为电喷雾离子源（ESI），负离子模式下采用飞行时间质谱采集数据，全扫描质量范围为m/z 100~1500。依据保留时间、精确分子量、二级质谱裂解碎片，结合对照品比对初步鉴定化合物。结果?从黄芪-丹参药对中初步鉴定或推断出28个化合物，其中丹参中15个化合物，黄芪中11个化合物，另有2个未知化合物。包括皂苷类、有机酸类、黄酮类和醌类化合物。结论?该UFLC-Q-TOF/MS可全面、快速分析黄芪-丹参药对中的化学成分，研究结果为对其进行活性成分研究和质量控制提供了一定参考。 

Analysis of the Main Components in Herbal Pair: Astragali Radix and Salviae Miltiorrhizae by UFLC-Q-TOF/MS

OBJECTIVE? To identify the main chemical components in herbal pair:Astragali Radix and Salviae Miltiorrhizae by UFLC-Q-TOF/MS. METHODS?Thermo BDS C₁₈ (3μm，150mm×2.1mm) column was employed for the separation with acetonetrile-water (0.1% formic acid) as mobile phase using a gradient elution program. The flow rate was set as 0.3mL/min， the temperature of column was 40℃ with injection volume of 5μL. Electrospray ionization (ESI)-Q-TOF/MS in negative ion mode was performed to obtain the MS spectra with full mass scan m/z ranged from 100 to 1500. Chemicals were identified based on the retention time， high-resolution mass information， MS/MS fragmentation behaviors and standards. RESULTS?28 components were identified or tentatively characterized in herbal pair:Astragali Radix and Salviae Miltiorrhizae， including 15 components in Salviae Miltiorrhizae， 11 compounds in Astragali Radix， and 2 unknown compounds. And these chemicals could be classified as triterpenes， organic acids， flavonoids and quinines. CONCLUSION?This method can rapidly and accurately identify the main compounds in herbal pair:Astragali Radix and Salviae Miltiorrhizae， which can help us for the quality control and further phytochemical studies of Astragali Radix and Salviae Miltiorrhizae. in traditional Chinese medicine.