竹节香附素A对人胃癌细胞株BGC-823细胞周期及STAT3信号通路的影响

目的?研究竹节香附素A(Raddeanin A，RA)在体外对胃癌细胞BGC-823的周期阻滞作用及对STAT3信号通路的影响。方法?应用MTT法检测RA对人低分化细胞BGC-823细胞的增殖影响，应用流式细胞仪检测RA对BGC-823细胞周期的阻滞作用，细胞划痕实验检测RA对BGC-823迁移、侵袭能力的影响，应用Western blot检测其对STAT3信号通路的影响。结果?RA在给药12、24、48h后，与对照组相比，对BGC-823细胞的增殖抑制有显著效果，呈现时间-浓度依赖关系。流式细胞周期结果显示，RA作用于人胃癌细胞BGC-823 12h后，S期比例与对照组相较显著上升，而G₂/M期比例下降，表明细胞被阻滞于S期。在浓度为8、16μmol/L时，镜下显示迁移过去的胃癌细胞数目均明显低于对照组，Western blot结果显示RA能下调p-STAT3蛋白的表达。结论?RA能有效抑制人胃癌细胞的增殖并阻滞其细胞周期于S期，并且抑制STAT3信号通路中p-STAT3的表达。 

The Effects of Raddeanin A on Human Gastric Cancer BGC-823 Cell Cycle and STAT3 Signaling Pathway

OBJECTIVE?To investigate the effects of Raddeanin A（RA） on cell cycle and STAT3 signaling pathway in human gastric cancer cell line BGC-823. METHODS?Effects of different concentrations of RA on proliferation of BGC-823 cell line were investigated with MTT assay; flow cytometry was used to detect the cell cycle inhibition; wounding heal assay was used to measure the influence of migration ability of RA on BGC-823 cell; the effect of STAT3 signaling pathway of RA on BGC-823 cell was measured by Western blot. RESULTS?After the BGC-823 cells were treated by RA for 12， 24， 48h， compared with control group， RA significantly inhibited the proliferation of BGC-823 cell (P<0.01). The inhibitive effects presented a dose and time-dependent manner. Flow cytometry demonstrated that RA blocked the cell cycle in S phase; wounding heal assay revealed that RA inhibited the migration of BGC-823 cell. Western blot results showed that RA down-regulated the expression of phosphorylated STAT3 (p-STAT3). CONCLUSION?RA can effectively inhibit the proliferation of BGC-823 cell， block its cell cycle in S phase and inhibit the expression of p-STAT3.