| 细胞凋亡荧光染色法检测肿瘤化疗药物敏感性并与MTT法的比较 |
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| 引用本文： | 童善庆,张希衡,朱佑明,胡宝瑜,丁建青,陆德源,钟晓松. 细胞凋亡荧光染色法检测肿瘤化疗药物敏感性并与MTT法的比较[J]. 中国肿瘤生物治疗杂志, 1997, 4(2): 125-129 |
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| 作者姓名： | 童善庆 张希衡 朱佑明 胡宝瑜 丁建青 陆德源 钟晓松 |
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| 作者单位： | 上海第二医科大学微生物学教研室肿瘤生物治疗实验室!上海200025;上海第二医科大学微生物学教研室肿瘤生物治疗实验室!上海200025;上海第二医科大学微生物学教研室肿瘤生物治疗实验室!上海200025;上海第二医科大学微生物学教研室肿瘤生物治疗实验室!上海200025;上海第二医科大学微生物学教研室肿瘤生物治疗实验室!上海200025;上海第二医科大学微生物学教研室肿瘤生物治疗实验室!上海200025;上海第二医科大学微生物学教研室肿瘤生物治疗实验室!上海200025 |
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| 摘 要： | 本文根据化疗药物诱导肿瘤细胞产生凋亡的机制,采用细胞凋亡荧光染色法(AFS)分别检测了卵巢癌细胞株(3AO)、部分肺癌患者分离的癌细胞药物敏感性,并和传统的ATT法进行比较.用不同浓度的顺铂诱导3AO凋亡、两种方法学之间没有显著性差别(P>0.05);但用于肺癌患者分离的肿瘤细胞进行药物的筛选时,有显著性差别(P
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| 关 键 词： | 抗肿瘤药物敏感试验 细胞凋亡荧光染色法 MTT法 |
| 收稿时间： | 1996-12-17 |
| 修稿时间： | 1997-02-11 |
Comparison of Apoptosis Fluorescence Staining Assay and MIT Assay in the Antitumor Drugs Sensitivity Test |
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| Tong Shanqing,Zhang Xiheng,Zhu Youming,Hu Baoyu,Ding Jianqing,Lu Deyuan and Zhong Xiaosong. Comparison of Apoptosis Fluorescence Staining Assay and MIT Assay in the Antitumor Drugs Sensitivity Test[J]. Chinses Journal of Cancer Biotherapy, 1997, 4(2): 125-129 |
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| Authors: | Tong Shanqing Zhang Xiheng Zhu Youming Hu Baoyu Ding Jianqing Lu Deyuan Zhong Xiaosong |
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| Affiliation: | Department of Microbiology , Cancer Biotherapy Laboratory , Shanghai Second Medical University, Shanghai 200025;Department of Microbiology , Cancer Biotherapy Laboratory , Shanghai Second Medical University, Shanghai 200025;Department of Microbiology , Cancer Biotherapy Laboratory , Shanghai Second Medical University, Shanghai 200025;Department of Microbiology , Cancer Biotherapy Laboratory , Shanghai Second Medical University, Shanghai 200025;Department of Microbiology , Cancer Biotherapy Laboratory , Shanghai Second Medical University, Shanghai 200025;Department of Microbiology , Cancer Biotherapy Laboratory , Shanghai Second Medical University, Shanghai 200025;Department of Microbiology , Cancer Biotherapy Laboratory , Shanghai Second Medical University, Shanghai 200025 |
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| Abstract: | Using in vitro established tumor cell lines as a target treated by the various concentrations cisplatium (DDP) and comparing with the conventionl MTT assay, we have set up an apoptosis fluorescence staining (AFS) in antitumor drugs sensitivity test. The results showed that there was no significant difiierence (P > 0.05) both in AFS assay and MTT assay to detect the apoptosis of the cell lines. However, there was significant difference ( P < 0.05) of the cytotoxic effect of the tumor cells derived from clinical samples. AFS assay is a rapid, accurate, reproductive and sensitive approach, which can observe three kinds of cell state i.e. apoptosis, necrosis and live cells at the same time. This method avoided the contaminated cells, cell fractions as well as the influence of other factors in MTT assay. It is possible to replace MTT assay for measuring cytotoxicity of antitumor drugs. |
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| Keywords: | antitumor drug sensitidy test apoptosis fluorescence staining(AFS) assay MTT assay |
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