双歧杆菌对高脂饮食诱导的C57BL/6小鼠非酒精性脂肪肝的影响

目的 探讨双歧杆菌是否通过改变肠道菌群，减少肠道局部炎症，调节系统性炎症，从而减轻高脂饮食诱导的C57BL/6小鼠的非酒精性脂肪性肝病（non-alcoholic fatty liver disease, NAFLD）。方法 将32只雄性C57BL/6小鼠作为实验动物，分为对照饲料组、对照饲料加双歧杆菌干预组、高脂饲料组和高脂饲料加双歧杆菌干预组，每组分2笼，每笼4只。检测小鼠血清糖脂代谢及肝功能水平。通过油红O染色和苏木精-伊红（H-E）染色评估小鼠肝脏脂肪变性情况。采用实时荧光定量PCR检测小鼠回肠、结肠组织中肿瘤坏死因子-α(TNF-α)、白细胞介素6(IL-6)和肠黏膜闭锁小带蛋白1(zonula occludens 1,ZO-1)、紧密连接蛋白（occludin）的表达。通过Illuminate Miseq平台微生物多样性测序检测小鼠粪便中肠道菌群的结构变化。结果 高脂饲料加双歧杆菌干预组C57BL/6小鼠肝脏脂肪变性程度明显低于高脂饲料组。与高脂肪饲料组相比，高脂饲料加双歧杆菌干预组炎症因子TNF-α、IL-6表达明显下降，肠道炎症减轻。与对照饲料组相比，高脂饲料组肠道菌群...

Effects of bifidobacteria on high-fat diet-induced non-alcoholic fatty liver disease in C57BL/6 mice

Objective: The objective of this study was to identify the role of bifidobacteria via targeting gut microbiota in NAFLD.Methods: Male C57BL/6 mice were fed either a normal-chow diet or a high-fat diet(HFD). bifidobacteria was administered orally by gavage to mice for 12 weeks. Gut microbiota profiles were established through 16SrRNA amplicon sequencing.The effects of bifidobacteria on liver inflammation, the gut barrier, gut microbiome were also investigated.Results: Bifidobacteria treatment can significantly improve obesity, circulation metabolism disorders,liver inflammation and fibrosis, and intestinal barrier damage caused by HFD. The gut microbial structure was changed, and abundance of Firmicutes was increased significantly after treated with bifidobacteria. Conclusion:Taken together,these data suggest that Bifidobacteria has aprotective effect on NAFLD via changing the components of gut microbiota.