高糖条件下大鼠下颌骨成骨细胞的活性

背景：临床研究证实，糖尿病是引起牙周病变及牙槽骨丧失的危险因素，而糖尿病所致的高血糖对颌骨改变的影响机制目前仍未完全阐明。 目的：观察高糖对体外培养的下颌骨成骨细胞增殖、分化、矿化的影响。 方法：分离培养大鼠下颌骨成骨细胞，分别在生理糖浓度(5.5 mmol/L；对照组)和高糖条件下(16.5 mmol/L；高糖组)培养，采用MTT法、PNPP法、生化法、放射免疫法及茜素红S钙染法检测各组细胞的增殖能力、碱性磷酸酶活性、钙吸收、骨钙素分泌水平及矿化能力。 结果与结论：高浓度的葡萄糖能显著促进成骨细胞的增殖(P < 0.05)，降低成骨细胞骨钙素的分泌(P < 0.01)，增加钙结节的数量及面积(P < 0.01)。与对照组比较，高糖组成骨细胞碱性磷酸酶活性在21 d时显著增高(P < 0.01)，钙吸收在14~21 d时显著降低，而在21~28 d时显著增高(P < 0.01)。说明高糖能促进体外培养的大鼠下颌骨成骨细胞的增殖，延迟其分化和矿化。

Effect of hyperglycemia on activity of rat mandibular osteoblasts

BACKGROUND: Clinical research has demonstrated that diabetes mellitus is a risk factor for periodontal lesion and alveolar bone loss. However, it remains poorly understood the effect of hyperglycemia on changes of jaw bone. OBJECTIVE: To investigate the effects of hyglycemia on proliferation, differentiation and mineralization of osteoblasts from rat mandible. METHODS: Primary osteoblasts were isolated and incubated with medium containing 5.5 mmol/L and 16.5 mmol/L glucose, respectively. Cell proliferation, alkaline phosphatase (ALP) activity, calcium uptake, bone gla protein (BGP) and mineralization were detected using MTT, PNPP, biochemistry, radioimmunoassay, as well as Alizarin Red S staining. RESULTS AND CONCLUSION: High glucose could significantly increase cell proliferation (P < 0.05), decreased BGP level (P < 0.01), and increase number and the area of nodules (P < 0.01). Compared with the control group, the ALP activity of the high glucose group was obviously increased at 21 days after culture (P < 0.01); the calcium uptake was decreased at 14-21 days, but notably increased at 21-28 days (P < 0.01). These findings suggest that diabetes-associated hyperglycemia promotes rat mandibular osteoblasts proliferation while delaying differentiation and mineralization.