凝结芽孢杆菌活菌TBC169株对右旋葡聚糖硫酸钠引发大鼠溃疡性结肠炎的治疗作用

目的:研究凝结芽胞杆茵(Bacillus coagulans,BC)对右旋葡聚糖硫酸钠(DSS)引发的大鼠溃疡性结肠炎(UC)的治疗作用.方法:采用DSS引发大鼠UC,分别用10~7和10~6 CFU/mL BC、0.02g/mL柳氮磺胺吡啶(SASP)和生理盐水(NS)处理.21d后测定动物体质量、结直肠湿重、肠黏膜溃疡、糜烂点数及面积、髓过氧化物酶活性和肠道菌群培养.结果:治疗21d后,各组中大鼠的体质量增加,以BC10~7 CFU/mL增重明显(P<0.05);各治疗组中大鼠的结直肠湿重、肠溃疡糜烂点数、面积、MPO活性与模型组比较均有显著性差异(P<0.05或P<0.01或P<0.001);肠道菌群分析,造型后双歧杆菌数明显下降(P<0.05),治疗后各组的双歧杆菌数量均明显增加(P<0.01或P<0.001),以BC组和SASP组增加最明显(P<0.01或P<0.001).BC在肠道定植.结论:BC对DSS引发的大鼠UC有明显治疗作用.

Therapeutic effects of Bacillus coagulans on the dextran sodium sulfate-induced ulcerative colitis in rats

AIM:To investigate the effect of Bacillus coagulans(BC,TBC169 strain)in the treatment of rats with ulcerative colitis induced by dextran sodium sulfate(DSS). METHODS:DSS was used to induce UC in Sprague Dawley rats,and then the rats were treated with 10~7 CFU/mL,10~6 CFU/mL BC,0.02 g/mL sulfasalazine and normal saline(control), respectively.After 21 days of treatment,the body weight,colorectal wet weight,intestinal mucosal ulcer,erosion spots and area,myeloper- oxidase(MPO)activity,and intestinal bacterial flora were observed. RESULTS:After treatment,the body weights of rats in all the groups were increased,especially in the group received BC at 10~7 CFU/mL(P<0.05).Intestinal wet weight,ulcerative number and area,and MPO activity were significantly reduced in all the treatment groups(P<0.05 or P<0.01 or P<0.001)as compared with those in model control group.Analysis on intestinal flora showed that the number of Bifidobacterium was decreased remarkably after modeling,but it was significantly increased in all the treatment groups(P<0.01 or P<0.001).BC was found colonized in the intestinal tract of BC-treated group. CONCLUSION:BC has notable therapeutic ef- fect on DSS-induced UC.