太行山区不同产地连翘中连翘苷、连翘酯苷A的含量测定

目的：采用HPLC法测定太行山区不同产地、不同采集时间连翘中连翘苷及连翘酯苷A的含量。方法：采用Agilent ZORBAX Eclipse XDB-C18（250mm×4.6mm，5μm）色谱柱，测定连翘苷以乙腈-水（25∶75）为流动相，等度洗脱；流速为1.0ml·min-1；检测波长为277nm。测定连翘酯苷A以乙腈-0.4％冰醋酸溶液（15∶85）为流动相，等度洗脱，流速为1.0ml·min-1；检测波长为330nm。结果：连翘苷和连翘酯苷A线性范围分别为0.206～2.060μg和0.120～1.200μg，平均回收率分别为99.52%（RSD=1.74%）和99.63%（RSD=1.21%）。结论：太行山区不同产地连翘中连翘苷和连翘酯苷A含量差异较大，7月份采集连翘中连翘苷、连翘酯苷A含量最高。含量测定方法准确、快速，适用于连翘的质量控制。

Determination of contents of phillyrin and forsythiaside A in Forsythia suspensa from Different Sites in Taihang mountain

Objective：To establish an HPLC method for the determination of phillyrin and Forsythia-side A in Forsythia suspense from different sites and gathering periods in Taihang mountain. Methods：Agilent ZORBAX Eclipse XDB-C18 （250mm×4.6mm,5μm） was applied. For determina-tion of phillyrin,Mobile phase was with acetonitrile-water （25∶75）, the separation was carried out with a gradient program at a flow rate of 1.0ml·min-1 and detection wavelength was 277nm. For determination of Forsythoside A, Mobile phase was with acetonitrile-0.4%acetic acid Solution（15∶85）,the separation was carried out with a gradient program at a flow rate of 1.0ml·min-1 and detection wavelength was 330nm. Results：Phillyrin and Forsythiaside A showed good linearity in the ranges of 0.206~2.060μg and 0.120~1.200μg with average recoveries of 99.52% and 99.63%,Respectively. Conclusions：The contents of phillyrin and Forsythiaside A in Forsythia suspensa from different sites in Taihang mountain marked difference, the contents of phillyrin and Forsythiaside A in Forsythia suspense was the highest in July,the method is simple and rapid for determination the contents of phillyrin and Forsythiaside A in Forsythia suspense,and can be used as the quality control of Forsythia suspensa.