紫外可见分光光度法测定叠鞘石斛中联苄类化合物的含量 |
| |
引用本文: | 贾芳,夏厚林,黄萍,黄小燕,宁梓君,唐建军,张廷模. 紫外可见分光光度法测定叠鞘石斛中联苄类化合物的含量[J]. 成都中医药大学学报, 2014, 37(1): 7-10 |
| |
作者姓名: | 贾芳 夏厚林 黄萍 黄小燕 宁梓君 唐建军 张廷模 |
| |
作者单位: | 贾芳 (成都中医药大学药学院,四川成都,611137); 夏厚林 (成都中医药大学药学院,四川成都,611137); 黄萍 (成都中医药大学药学院,四川成都,611137); 黄小燕 (成都中医药大学药学院,四川成都,611137); 宁梓君 (成都中医药大学药学院,四川成都,611137); 唐建军 (成都中医药大学药学院,四川成都,611137); 张廷模 (成都中医药大学药学院,四川成都,611137); |
| |
基金项目: | 科技部“十二五”支撑计划项目(项目编号:2011BAI13BO2-8) |
| |
摘 要: | 目的:建立叠鞘石斛中联苄类化合物的含量测定方法。方法:以石斛酚为对照品,未加显色剂条件下,建立紫外可见分光光度法测定叠鞘石斛中联苄类化合物的含量。结果:确定最佳吸收波长280nm,石斛酚在浓度4.88-58.56μLg/mL范围内呈良好线性关系(r=0.9998),平均加样回收率为97.39%,RSD=2.97%。结论:该方法准确、简单、快捷,重现性好,适用于叠鞘石斛中联苄类化合物含量的测定。
|
关 键 词: | 叠鞘石斛 石斛酚 联苄类化合物 含量测定 紫外可见分光光度法 |
Determination of Bibenzyl Compounds Content in Dendrobinm denneanum by UV-Visible Spectrophotometry |
| |
Affiliation: | JIA Fang, XIA Hou-lin, HUANG Ping, et al ( Chengdu University of Traditional Chinese Medicine, Chengdu, 611137, China) |
| |
Abstract: | Objective: To establish the content determination method for bibenzyl compounds in Dendrobinm denneanum. Methods: Under the condition of not adding chromogenic agent, using ultraviolet and visible spectmphotometfic determi- nation to detect the content of bibenzyl compounds with gigantol as the standard substance. Results: The best absorption wavelength was 280 nm. Gigantol had a liner relationship with the absorbance in the range of 4. 88 - 58. 56 p.g/mL ( r = 0. 9998 ) . The average re- covery for gigantol was 97. 39%, RSD was 2.97%. Conclusion: The method is simple, sensitive, accurate and can be used for bibenzyl compounds determination in Dendrobinm denneanum. |
| |
Keywords: | Dendrobinm denneanum gigantol bibenzyl compounds determination of contents UV-visible spectrophotometry |
本文献已被 维普 等数据库收录! |
|