组蛋白去甲基化酶KDM6A促进骨髓间充质干细胞成骨分化研究

目的研究组蛋白去甲基化酶KDM6A对骨髓间充质干细胞成骨定向分化能力的影响。方法构建Flag-KDM6A慢病毒表达质粒。利用慢病毒转染构建稳定过表达KDM6A的骨髓间充质干细胞。碱性磷酸酶活性实验检测成骨分化早期指标-碱性磷酸酶活性。茜素红染色及钙离子定量分析检测干细胞体外矿化能力。实时：毫量RT-PCR检测成骨分化相关基因．骨桥蛋白和骨钙素的表达。结果过表达KDM6A促进骨髓间充质干细胞碱性磷酸酶活性、骨髓间充质干细胞体外矿化能力以及骨桥蛋白和骨钙素的表达。结论组蛋白去甲基化酶KDM6A具有促进骨髓间充质干细胞成骨分化的潜能，可以作为一个候选的靶基因用于骨组织工程技术，促进骨组织再生。．

Histone demethylase KDM6A enhanced the osteogenic differentiation potential of bone marrow mesenchymal stem

Objective To investigate the effect of histone demethylase lysine （K）-specific demethylase 6A （KDM6A） on the osteogenic differentiation potential of bone marrow mesenehymal stem cells （BMSCs）. Methods Lentiviral Flag-KDM6A was used to establish the stable cells and over-expressed KDM6A for Gain-of-function study in BMSCs. Alkaline phosphatase （ALP） activity assay was used to detect the early marker of osteogenic differentiation-ALP activity. Alizarin-red staining and quantitative analysis of calcium were used to investigate the mineralization potential of BMSCs in vitro. The expressions of osteogenesis related genes osteopontin （OPN） and osteocalcin （OCN）were examined by ：real time RT-PCR. Results The over-expression of KDM6A enhanced ALP activity, mineralization, and the expression of OPN and OCN in BMSCs. Conclusion Histone demethylase KDM6A promoted osteogenic differentiation potential of BMSCs and could be used as a candidate target in regeneratinz bone tissue with en~ineerinz technology.