| NCTD对白血病细胞DR5表达的影响 |
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| 引用本文： | 李妍,张铎,方芳,陈爽,赵良中. NCTD对白血病细胞DR5表达的影响[J]. 吉林医药学院学报, 2014, 35(1): 5-7 |
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| 作者姓名： | 李妍 张铎 方芳 陈爽 赵良中 |
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| 作者单位： | 李妍 (吉林医药学院检验学院,吉林,吉林,132013); 张铎 (吉林医药学院检验学院,吉林,吉林,132013); 方芳 (吉林医药学院检验学院,吉林,吉林,132013); 陈爽 (吉林医药学院检验学院,吉林,吉林,132013); 赵良中 (吉林医药学院检验学院,吉林,吉林,132013); |
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| 基金项目： | 吉林省教育厅"十二五"科学技术研究项目(项目编号：2011420)吉林省科技厅中青年科技创新领军人才及团队项目(项目编号：20130521018JH)国家自然科学基金项目(项目编号：82102953) |
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| 摘 要： | 目的 研究去甲斑蝥素（NCTD）对白血病细胞死亡受体（DR）5表达的影响.方法不同浓度NCTD处理K562和HL-60细胞16 h后,流式细胞术分析DR5表达和细胞内活性氧（ROS）相对含量;通过免疫印迹技术分析激酶JNK1的表达.结果 NCTD以浓度依赖方式上调DR5表达,细胞内活性氧含量和JNK1表达也随NCTD浓度增大而增加.结论 NCTD可能通过ROS/JNK途径上调DR5表达.
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| 关 键 词： | 去甲斑蝥素 活性氧 肿瘤坏死因子相关的凋亡诱导配体 死亡受体5 |
The study of DR5 on leukemia cells induced by NCTD |
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| LI Yan,ZHANG Duo,FANG Fang,CHEN Shuang,ZHAO Liang-zhong. The study of DR5 on leukemia cells induced by NCTD[J]. Journal of Jilin Medical College, 2014, 35(1): 5-7 |
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| Authors: | LI Yan ZHANG Duo FANG Fang CHEN Shuang ZHAO Liang-zhong |
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| Affiliation: | ( School of of Medical Laboratory, Jilin Medi- cal College, Jilin City, Jilin Province, 132013, China) |
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| Abstract: | Objective To explore the expression of DR5 on leukemia cells induced by NCTD. Methods Leukemia cells K562 and HL-60 were cultured in vitro and treated by different concentration of NCTD for 16 hours. The expres- sion of DR5 and ROS in cells were measured by flow cytometry, and the content of JNK1 in cells was detected by west- ernblot assay. Results It was found that NCTD could up-regulated DR5 on leukemia cells in a dose-dependent man- ner. Both the levels of ROS and JNK1 rise with increasing in the concentration of NCTD. Conclusion The expres- sion of DR5 on leukemia cells could be up-regulated by NCTD through ROS/JNK signaling pathway. |
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| Keywords: | NCTD ROS TRAIL DR5 |
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