睡眠剥夺对HSP70表达及脑超微结构的影响

目的：观察睡眠剥夺（SD）后大鼠脑组织HSP70表达的变化及对超微结构的影响。方法：44只雄性SD大鼠随机分为11组，每组4只，免疫组织化学方法检测HSP70的表达，电镜观察海马超微结构的变化。结果：睡眠剥夺后12小时即可在大脑皮质及海马观察到HSP70阳性细胞，2—3天数量达到高峰，7天时明显下降。白天睡眠剥夺12小时（SDd12h）组HSP70阳性细胞数较夜晚睡眠剥夺12小时（SDn12h）组多（P〈0．05）。RS组大脑皮质HSP70阳性细胞数较白天睡眠剥夺1天（SDd1d）组减少（P〈0．05）。白天睡眠剥夺3天（SDd3d）海马出现超微结构改变，白天睡眠剥夺7天（SDd7d）后改变更加明显。结论：睡眠剥夺可影响大鼠脑组织HSP70表达及超微结构。

Change of heat shock protein 70 expression and ultrastructure of brain in sleep deprivation

Objective:To study the change of the expression of heat shock protein 70 and ultrastructure of brain in sleep deprivation(SD).Methods:44 male Sprague-Dawlay rats were randomly divided into 11 groups.The HSP70 positive cells were detected by using immunohistochemical methods.The ultrastructure of hippocampal neuron was observed through electromicroscope.Results:HSP70 were detected in the cerebral cortex and hippocampus after SD.The HSP70 could be found after 12 h SD,reached to a peak during 2-3 d SD,and declined after 7 d SD.The number of HSP70 positive cells of SDd12h group was markedly more than that of SDn12h group(P<0.05).The number of HSP70 positive cells of RS group was markedly fewer than that of SDd1d group in cerebral cortex(P<0.05).The ultrastructure of hippocampal neuron was damaged after 3 d SD,and the damaged was more severe after SDd7d.Conclusion:Sleep deprivation may affect HSP70 expression and the ultrastructure of hippocampal neuron.