肝郁气滞型乳腺增生症病变组织中ER与ERmRNA的表达

研究雌激素受体 ( ER)与受体基因 ( ERm RNA)表达在乳腺增生病辨证分型中的变化及其意义 ,采用免疫组化法和逆转录 -聚合酶链反应法 ( RT-PCR) ,检测正常乳腺和乳腺增生组织中 ER与 ERm RNA的表达 ,结果显示肝郁气滞型组 ER与 ERm RNA表达均显著高于正常对照组 ,但低于冲任失调组 ;2组增生组织中 ER和 ERm RNA表达之和与正常组对比有非常显著差异。说明 ER、ERm RNA高表达导致乳腺组织对雌激素的敏感性增强可能是乳腺组织增生的重要因素之一 ,ER与 ERm RNA表达水平的差异性可作为临床辨证分型的客观指标。

The Expression of ER and ERmRNA in Mammary Hyperplasic Tissue in Stagnation of Liver-qi Type

Subject:to study the change and importance of the expression of estrogen receptor(ER)?estrogen receptor gene(ERmRNA)in syndrome differentiation of mammary hyperplasia.Method:the expression of ER was tested by immunohistochemical assay(S P),ER gene expression was tested by RT PCR in healthy mamary tissue and hyperplasic tissue.Results:the expression of ER and ERmRNA expression in stagnation of Liver qi type were higher than those in healthy control group significantly,but lower than those in disorder of Thoroughfare Vessel and Conception Vessel type significantly.The expression of ER and ERmRNA in hyperplasic tissue was higher than that of healthy tissue,the former significantly.Conclusion:the high expression of ER and ERmRNA,the enhanced sensitivity of estrogen maybe one of the important causes.The expression level of ER and ERmRNA can be regarded as objective indexes of syndrome differentiation in clinic.