Elevated sperm DNA fragmentation does not predict recurrent implantation failure |
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| Authors: | Jordan C. Best BS Taylor Kohn MD Premal Patel MD Ruben Blachman-Braun MD Elaine de Quadros PhD Zeki Beyhan PhD Michael Jacobs MD Ranjith Ramasamy MD |
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| Affiliation: | 1. Department of Urology, University of Miami Miller School of Medicine, Miami, FL, USA;2. Department of Urology, Johns Hopkins University School of Medicine, Baltimore, MD, USA;3. Section of Urology, Department of Surgery, University of Manitoba;4. Fertility & IVF Center of Miami, Miami, FL, USA |
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| Abstract: | In this study, we sought to determine whether sperm DNA fragmentation (DFI%) and high DNA stainability (HDS%) evaluated by sperm chromatin structure assay (SCSA) predict recurrent implantation failure (RIF) or pregnancy rate. A retrospective study was performed of consecutive cycles of ICSI treatment from 2009 to 2018. A total of 386 couples that underwent 1,216 frozen embryo transfer (FET) cycles were analysed. Mean female and male age was 34 ± 3.6 years and 37.3 ± 6.6 years, respectively, and a median total motile sperm count (TMSC) was 43.5 [9.9–105.5] million. Overall median DFI% and HDS% was 12 [7.1–18.9] and 9.6 [6.5–14.4] respectively. On multivariable analysis, DFI% and HDS% were not associated with RIF (DFI%: OR = 1.01, 95% CI: 0.98–1.04, p = .414; HDS%: OR = 0.97, 95% CI: 0.94–1.01, p = .107) or IVF success, defined as clinical pregnancy (DFI%: OR = 1.00, 95% CI: 0.99–1.01, p = .641; HDS%: OR = 1.01, 95% CI: 0.99–1.02, p = .565). We found that neither DFI% or HDS%, as assessed by SCSA, were predictive of RIF or pregnancy rate. This finding suggests that sperm DNA fragmentation does not predict RIF or pregnancy rate. |
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| Keywords: | DNA fragmentation high DNA stainability in vitro fertilization intracytoplasmic sperm injection recurrent implantation failure |
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