Cholesterol-loaded cyclodextrin attenuates dilution effect and improves quality of bovine low sperm insemination doses during cryopreservation |
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Authors: | Shabir Ahmad Lone Tushar Kumar Mohanty Mukesh Bhakat Hanuman Prasad Yadav Adil Rasool Paray Raju Kumar Dewry Rubina Kumari Baithalu Ranjana Sinha Pradeep Kumar |
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Affiliation: | 1. Animal Reproduction, Gynecology & Obstetrics, ICAR-National Dairy Research Institute, Karnal, India;2. Livestock Production and Management (LPM), ICAR-National Dairy Research Institute, Karnal, India;3. Animal Physiology and Reproduction Division, ICAR-Central Institute for Research on Buffaloes, Hisar, India |
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Abstract: | In the present study, the effect of cholesterol-loaded cyclodextrin (CLC) on the quality of low sperm doses at post-thaw was evaluated. Twenty four ejaculates (6 from each bull) were collected and split into eight aliquots. First four aliquots were diluted up to 20-, 15-, 10- and 5-million sperm/0.25 ml, and remaining four were treated with CLC at the rate of 1 mg/120 million spermatozoa, followed by dilution up to 20-, 15-, 10- and 5-million sperm/0.25 ml. The diluted semen was equilibrated, cryopreserved and evaluated post-thaw. The averages of total motility, progressive motility, average path velocity, straight linear velocity, membrane intact spermatozoa and noncapacitated spermatozoa were higher (p < .05) in CLC-treated sperm doses compared to control ones. However, the moribund spermatozoa, capacitated spermatozoa and acrosome-reacted spermatozoa were reduced (p < .05) in CLC-treated spermatozoa compared to control. The curvilinear velocity and linearity did not differ (p > .05) between control and CLC-treated sperm doses. In conclusion, treatment of spermatozoa with CLC at the rate of 1 mg/120 million spermatozoon attenuates the dilution effect and improves the quality of bovine low sperm insemination doses during cryopreservation; hence it could be a favourable cryoprotectant for preserving bovine semen at higher dilutions. |
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Keywords: | cholesterol-loaded cyclodextrin dilution effect post-thaw sperm quality |
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